How does the brain encode sensory information? To approach its answer, we use a mechanosensory system of the fruit fly as a model system. Fruit flies respond behaviorally to sound, gravity, and wind. Johnston's organ (JO), the antennal ear of the fly, serves as a sensory organ to detect these mechanosensory stimuli. Specific subgroups of sensory neurons in JO, so-called JO neurons subgroups, work as sensors for vibrations and static deflections of the antennal receiver. Five subgroups of JO neurons, subgroups A to E, reportedly project to one of the five AMMC zones, zones A to E, in the brain. Despite intensive analyses on the functional role of these subgroups, the function of subgroup-D JO neurons, which show unique anatomic characteristics when compared with other subgroups of JO neurons, is little understood. Here, we explored the physiologic properties of subgroup-D JO neurons by using GCaMP3-based calcium imaging. In contrast to other subgroups of JO neurons, all of which were reportedly activated either by vibrations (subgroups A and B) or by static deflections (subgroups C and E), subgroup-D JO neurons responded to both stimuli; vibrating at around 100-200 Hz and anterior deflection of the receiver selectively evoked strong calcium response in subgroup-D JO neurons in the brain. This finding clearly revealed that subgroup-D JO neurons could encode the position and movement of the antennal receiver. The five anatomically defined zones as projection targets of JO neurons are now defined as three functionally distinct groups: (1) a primary vibration center (zones A and B), (2) a primary deflection center (zones C and E), and (3) a primary vibration and deflection center (zone D). A fly brain thus could encode information about complex movements of the antennal receiver by expanding its movement into 5-dimensional space in the fly brain.