Magnetic stimulation is one of technique to activate central nervous and peripheral nervous systems non-invasively. We previously reported that volitionally controlled trans-vertebral magnetic stimulation (TVMS) to the lumbar vertebra could activate spinal neural circuit producing walking behavior of legs. Although the TVMS around lumbar vertebra may activate autonomic system as well as motor and sensory systems, it is largely unknown how the effect to the autonomic system is brought by the TVMS. To ensure the safety of participants, we recorded vital signs during TVMS. TVMS was applied on lumbar vertebra. The intensity of TVMS was 60 % of maximum output of stimulator. The frequency was modulated between 1 to 18 Hz. The neurologically-normal participants (n=23) were asked to control the frequency of TVMS by the level of finger muscle activity to induce walking-like behavior. Heart rate and SpO2, were recorded before, during and after TVMS. Blood pressure as recorded before and after TVMS. Participants were also asked to report a feeling if they feel some discomfort or pain sensation by the TVMS at a stimulation site. There were no change of heart rate and SpO2 before, during and after TVMS. The TVMS did not also induce modulation of blood pressure. Participants did not report the discomfort and pain feeling by TVMS around L2-L3 intervertebral which was hot spot inducing walking behavior, although they reported discomfort and pain feeling when the TVMS was applied more caudal or rostral part of lumbar vertebra. Participants reported discomfort and pain feeling as intensity of magnetic stimuli increased. These results suggest that TVMS to the lumbar vertebral do not induce serious problem in autonomic system. TVMS around L2-L3 intervertebral is optimal site to induce walking behavior without discomfort.