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演題詳細

Poster

睡眠、生体リズム
Sleep and Biological Rhythms

開催日 2014/9/12
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

睡眠中の大脳皮質におけるカルシウム動態の2光子励起イメージング
Cortical calcium dynamics in naturally sleeping and waking mice

  • P2-215
  • 上田 壮志 / Takeshi Kanda:1 上田 なつ子 / Natsuko Kanda:1 石井 亮 / Ryo Ishii:1 柳沢 正史 / Masashi Yanagisawa:1,2 
  • 1:筑波大学 国際統合睡眠医科学研究機構 / International Institute for Integrative Sleep Medicine, University of Tsukuba, Ibaraki, Japan 2:テキサス大学サウスウエスタン医学センター、テキサス、米国 / University of Texas Southwestern Medical Center, TX, USA 

Sleep is controlled by the brain and essential not only for the rest of body, but also for the maintenance of brain functions. It is well known that the cortical states defined by electroencephalography (EEG) vary with the sleep stages of wakefulness, NREM sleep and REM sleep. In other words, cortex is most susceptible brain region to sleep. However it is unclear what happens in the cortex when animals sleep. Since EEG represents the summed electrical activity of a large number of cells in the cortex, it is impossible to estimate individual neuron activity from EEG. Clarifying the spatio-temporal activity patterns of cortical cells during sleep and wakefulness requires visualization of cortical activity with cellular resolution in naturally sleeping and awake animals. To achieve this, we developed the method for two-photon calcium imaging of cortical cells in sleeping mice. The mouse head was restrained with the head plate under the objective while the mouse could move limbs freely on the spherical treadmill (trackball). It took several days to acclimate mice to the surroundings. EEG and EMG were recorded using miniature screw electrodes and wire electrodes. Using this method, we performed calcium imaging in the layer 2/3 of primary motor cortex during wakefulness, NREM sleep and REM sleep. The spatio-temporal patterns of spontaneous calcium signals in neurons changed in response to the sleep stages. Our results suggest activity patterns of cortical neurons, both at individual level and at population level, depend on sleep and consciousness.

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