演題詳細
Poster
学習・長期記憶
Learning and Long-term Memory
開催日 | 2014/9/11 |
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時間 | 11:00 - 12:00 |
会場 | Poster / Exhibition(Event Hall B) |
匂い連合学習によって生じる匂い嗜好性変化のメカニズム
The mechanisms of learning dependent odor preference changes following olfactory conditioning
- P1-269
- 長野 慎太郎 / Shintaro Nagano:1 上野 耕平 / Kohei Ueno:1 川端 有紀 / Yuki Kawabata:2 齊藤 実 / Minoru Saitoe:1
- 1:(公財)東京都医学総合研究所 / Tokyo Metropolitan Institute of Medical Science 2:首都大学東京 / Tokyo Metropolitan University
Experience-dependent behavioral changes are important for animals to survive even in fruit fly Drosophila melanogaster. Olfactory conditioning is an example of such experience-dependent changes, and we use two aversive conditioning odors (CS) to condition flies; flies are sequentially exposed to the first CS+ odor with electrical shocks and the second CS- odor without electrical shocks.
Previously, we found two classes of memory components, memory for avoiding CS+ (CS+ memory) and memory for approaching CS- (CS- memory), after olfactory conditioning. Importantly, without CS+ memory formation, flies still avoid CS- odor, suggesting that significance of CS- odor for flies is changed by previous experience. In addition whereas CS+ memory was maintained more than 3hr, CS- memory was declined within 3hr.
In this study, we found that mutations in D1 type dopamine receptor (D1R) gene disrupt both CS+ and CS- memory formation. To explore the brain regions required for CS+ and CS- memory formation, we employed GAL4/UAS binary system, which allows us to manipulate D1R expression in specific brain regions. We found that D1R function in the mushroom bodies (MBs), neural center for olfactory memory formation, is necessary for CS+ memory but not for CS- memory. In contrast, D1R function in the projection neurons (PNs) in the antennal lobes (ALs), the primary olfactory center, is required for CS- memory but not for CS+ memory. These results suggest that although both CS+ and CS- memory require D1R activity they are formed in different brain regions.