演題詳細
Poster
受容体、輸送体
Receptors and Transporters
| 開催日 | 2014/9/11 |
|---|---|
| 時間 | 16:00 - 17:00 |
| 会場 | Poster / Exhibition（Event Hall B） |
Organic Cation Transporter 2 (SLC22A2) はコリン作動性ニューロンのシナプス小胞に優先的に局在している
Organic Cation Transporter 2 (SLC22A2), A low-affinity and high-capacity choline transporter, is preferentially enriched on synaptic vesicles in cholinergic neurons
- P1-026
- 中田 隆博 / Takahiro Nakata:1,2 松井 利康 / Toshiyasu Matsui:2 小林 久美子 / Kumiko Kobayashi:1 小林 靖 / Yasushi Kobayashi:2 安西 尚彦 / Naohiko Anzai:3
- 1:石川県立看護大学・健康科学 / Ishikawa Prefectural Nursing University, Ishikawa, Japan 2:防衛医科大学校・解剖 / Dept of Anat and Neurobiol, National Defense Medical College, Saitama, Japan 3:独協医科大学・薬理 / Dept of Pharmacol and Toxicol, Dokkyo Med Univ School of Medicine, Tochigi, Japan
Organic cation transporters (OCTs) are expressed mainly in the kidney and liver. OCTs transport intrinsic organic cations, including monoamine, dopamine, serotonine and choline, across the plasma membrane. Here, we demonstrate that OCT2 (SLC22A2) is expressed in cholinergic neurons, motoneurons in the anterior horn of the spinal cord, and is implicated in acetylcholine (Ach) recycling in presynaptic terminals. Application of rabbit antipeptide antibody revealed that OCT2 was expressed in the anterior horn of the spinal cord. Double immunostaining of muscle sections with anti-OCT2 and alpha-bungarotoxin (BTX) revealed that OCT2 was localized in the neuromuscular junctions (NMJs). Immunoelectron microscopy revealed that OCT2 was localized both in synaptic vesicles (SVs) in presynaptic terminals around the motoneurons (C-terminals) and in SVs in nerve terminals in NMJs. The similarity in the distribution of OCT2 in cholinergic neurons and that of vesicular acetyl choline transporter (VAchT), and the fact that OCT2 can transport choline suggest that OCT2 could work as a low-affinity and high-capacity choline transporter at presynaptic terminals in cholinergic neurons in a firingdependent manner.