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Neuroinflammation and Inflammatory Disease in Nervous System

開催日 2014/9/13
時間 15:00 - 16:00
会場 Room I(311+312)
Chairperson(s) 吉良 潤一 / Jun-ichi Kira (九州大学大学院医学研究院 神経内科学 / Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyusyu University, Japan)
錫村 明生 / Akio Suzumura (名古屋大学環境医学研究所 神経免疫 / Department of Neuroimmunology, Research Institute of Environmental Medicine, Nagoya University, Japan)

Atopic inflammation induces microglial activation and tactile allodynia

  • O3-I-3-2
  • 山﨑 亮 / Ryo Yamasaki:1 王 氷 / Bing Wang:2 吉良 潤一 / Jun-ichi Kira:2 
  • 1:九州大院・医・神経治療学 / Dept of neurology, Kyushu Univ.,Fac of Med., Japan 2:九州大・院・神経内科学 / Dept. Neurological therapeutics, Univ of Kyushu, Kyushu, Japan 

To elucidate the mechanism of tactile allodynia in patients with atopic myelitis using asthma model mice.
Six week-old male C57Black6 mice were used in this study. Fifty micrograms of ovalbumin (OVA) with 2 mg of aluminum hydroxide (Alum) dissolved in 200 μl of phosphate buffer (PBS) were intraperitoneally injected (i.p.) once a week for 3 times. In the third week, OVA 2.5 mg/ml were inhaled for 5 consecutive days to induce bronchial asthma model. Control groups (Alum i.p. and PBS i.p. groups) were also prepared.
The procedure was repeated for another three weeks (total 6 weeks). At the end of third weeks and sixth weeks, tactile allodynia was evaluated by the reaction rate of stimulation by von Frey filaments. After the behavioral tests, mice were sacrificed and subjected to the immunohistochemical analysis. Peripheral bloods were also collected for the evaluation of cytokine levels in each group.
Bronchial asthma were successfully induced confirmed by the robust eosinophilic infiltration in the lung. Tactile allodynia were observed in the allergic model mice, which were not in PBS control groups. In the spinal cord, microglial activation was seen mainly in dorsal horn region; the number was increased and also the ramification of microglia was reduced. These microglia expressed activation markers such as interferon regulatory factor 8 (IRF8).
We have found the microglial activation in allergic asthma model mice. These mice also developed tactile allodynia, simulates neuralgic pain / dysesthesia observed in patients with atopic myelitis. Since activation of microglia were reported to be related to the development of allodynia, elucidation of the relationship between peripheral allergic inflammation and microglial activation will be a clue to discover therapeutic options which benefit patients with atopic myelitis.

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