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演題詳細

Oral

成体ニューロン新生
Adult Neurogenesis

開催日 2014/9/12
時間 17:10 - 18:10
会場 Room J(313+314)
Chairperson(s) 影山 龍一郎 / Ryoichiro Kageyama (京都大学ウイルス研究所 / Institute for Virus Research, Kyoto University, Japan)
金子 奈穂子 / Naoko Kaneko (名古屋市立大学大学院医学研究科 再生医学分野 / Department of Developmental and Regenerative Biology, Nagoya City University Graduate School of Medical Sciences, Japan)

新生ニューロンの移動形態制御による嗅球内停止位置の決定
Activity-dependent layer-specific sorting of new neurons in the olfactory bulb by Sema3E-PlexinD1 signaling-mediated regulation of migratory morphology

  • O2-J-5-1
  • 澤田 雅人 / Masato Sawada:1 黄 詩惠 / Shih-hui Huang:1 匹田 貴夫 / Takao Hikita:1 植村 明嘉 / Akiyoshi Uemura:2 澤本 和延 / Kazunobu Sawamoto:1 
  • 1:名古屋市大院・医・再生医学 / Department of Developmental and Regenerative Biology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan 2:名古屋市大院・医・網膜血管生物学 / Department of Retinal Vascular Biology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan 

In the postnatal brain, new neurons are continuously generated in the ventricular-subventricular zone and migrate toward the olfactory bulbs (OB), a primary center for olfactory processing. In the OB, each new neuron is differentiated into one of two types of olfactory interneurons, granule cell and periglomerular cell, located in the granule cell layer (GCL) and glomerular layer (GL), respectively. Here we report that the Sema3E-PlexinD1 signaling plays a critical role in the maintenance of neuronal migration in the OB. We found that granule cells form "lateral processes" branched from the leading process before the termination of their migration in the GCL, while periglomerular cells maintain their bipolar morphology until they stop their migration at the GL. PlexinD1 expression is maintained in the bipolar-shaped migrating neurons, but downregulated by endocytosis in the lateral-process-bearing cells before their termination of migration. In vitro FRET and photoactivation experiments revealed that Sema3E-PlexinD1 signaling decreases Rac1 activity, thereby suppressing formation of lateral processes, leading to the maintenance of bipolar migratory morphology of new neurons. PlexinD1 expression in migrating new neurons regulates their migration and final positioning within the OB, which is affected by odor enrichment. Taken together, results suggest that maintenance and termination of migration of new neurons by Sema3E-PlexinD1 signaling determines their final destination layers in the OB in an activity-dependent manner.

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