The comparison of food consumption and locomotor activity between Taiwan native rodent, Formosan wood mice (Apodemus semotus), and laboratory mice, C57BL/6, were examined in this study. Therefore we examined the instinctive behavioural changes in different strains of mice to confirm the establishment of animal model for hyperactivity disorders. Using the behavioral indices of open field, elevated plus maze, hot plate and novel object recognition tests as the behavioral examinations was done in this study. The food consumption exhibited the circadian rhythmicity, i.e. higher during the lights-off period and lower during the lights-on period, in either Formosan wood mice or C57BL/6 mice. We also found that Formosan wood mice ate more food than C57BL/6 mice during the lights-off period and the whole day in male, but not in female. Similarly, the male Formosan wood mice had the higher locomotor activity than the male C57BL/6 mice during the lights-off period, but this phenomenon did not appear in female mice. These results indicate that even the Formosan wood mice successfully inbreed in the laboratory, they still keep more native paradigm than the laboratory C57BL/6 mice. This study is the first report to provide the basic physiological comparisons on native and common laboratory mice.