演題詳細
Poster
神経伝達物質、グリオトランスミッター、修飾物質
Neurotransmitters, Gliotransmitters, and Modulators
開催日 | 2014/9/11 |
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時間 | 11:00 - 12:00 |
会場 | Poster / Exhibition(Event Hall B) |
線条体の遅いカルシウム振動は直接路及び間接路ニューロンで発生している
Direct and indirect striatal projection neurons exhibited slow calcium oscillations
- P1-015
- 田村 篤史 / Atsushi Tamura:1 山田 尚弘 / Naohiro Yamada:2 矢口 雄一 / Yuichi Yaguchi:2 菊田 里美 / Satomi Kikuta:1 本間 経康 / Noriyasu Homma:1 小林 和人 / Kazuto Kobayashi:3 小山内 実 / Makoto Osanai:1
- 1:東北大院医 / Tohoku Univ Grad Sch Med, Miyagi, Japan 2:大阪大院工電気電子情報工学 / Grad Sch Eng, Osaka Univ, Osaka, Japan 3:福島県立医大医生体情報伝達研生体機能 / Dept Mol Gen, Inst Biomed Sci, Fukushima Med Univ, Fukushima, Japan
The calcium ion (Ca2+) is a universal and versatile signal transduction molecule, and plays a prominent role of defining the cellular state. There are many types of metabotropic receptors contributing to intracellular Ca2+ signaling. Metabotropic glutamate receptors (mGluRs) expressing abundantly in striatum also related to the Ca2+ signaling and are thought to be related to Parkinson's disease. However, it is not clear that the character of the Ca2+ signaling and which signal cascade contributes to that in the striatum. Here we show that both neurons and astrocytes of the striatum exhibited, the spontaneous slow Ca2+ oscillations, which lasted up to approximately 200 s. This slow Ca2+ oscillation was blocked by the depletion of the intracellular Ca2+ store and by an antagonist of inositol 1,4,5-trisphosphate (IP3) receptors. We found that mGluR5 decreased significantly the rate of the slow Ca2+ oscillation in both neurons and astrocytes. Thus, the mGluR5-IP3 signal cascade is the primary contributor to the slow Ca2+ oscillation in both neurons and astrocytes. To clarify the cell type of striatal neurons, we conducted the Ca2+ imaging study in the transgenic mice expressing yellow fluorescent protein (YFP) in D1 or D2 dopamine receptor (D1R or D2R)-positive neurons. In this study, we found the slow Ca2+ oscillations in both the D1R- and D2R-positive neurons in the striatum. This result suggested that striatal projection neurons participating direct and indirect pathway exhibited the slow Ca2+ oscillations.