• Top page
  • Timetable
  • Per session
  • Per presentation
  • How to
  • Meeting Planner



Molecular, Biochemical, and Genetic Techniques

開催日 2014/9/12
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Newly optical imaging systems of change in oxygen metabolism and hemodynamic using awake mice brain

  • P2-379
  • 田桑 弘之 / Hiroyuki Takuwa:1 西野 明日香 / Asuka Nishino:1,2 松浦 哲也 / Tetsuya Matsuura:1,2 坂田 和美 / Kazumi Sakata:2 田島 洋祐 / Yosuke Tajima:1 伊藤 浩 / Hiroshi Ito:1 
  • 1:独立行政法人 放射線医学総合研究所 / Molecular Imaging Center, National Inst of Radiological Sciences 2:岩手大学 工学部 応用化学・生命工学科 / Department of Chemistry and Bioengineering, Faculty of Engineering, Iwate University, Japan 

Positron-emission tomography (PET) allows the measurement of cerebral blood flow (CBF), cerebral blood volume (CBV) and cerebral metabolic rate of oxygen (CMRO2) in human and plays an important role in the diagnosis of pathologic conditions and clinical research. On the other hand, in animal studies, there is no optical imaging system for evaluating changes in CBF and CBV, and oxygen metabolism, from the same brain area under awake condition. In the present study, we developed a simultaneous measurement system of laser speckle imaging (LSI) and intrinsic optical signal imaging (IOSI), which was verified by laser-Doppler flowmetry (LDF). Moreover, to measure oxygen metabolism, flavoprotein autofluorescence imaging (FAI) was performed from the same brain area as LSI and IOSI measurements. The change in CBF according to LSI was correlated with that by LDF. Similarly, the change in CBV obtained by IOSI was also correlated with RBC concentration change measured by LDF. The change in oxygen metabolism by FAI was correlated with that in CBF obtained by LSI, although the change in CBF was greater than that in oxygen metabolism. We revealed that the relationship between oxygen metabolism and CBF as measured by our system was in good agreement with the relationship between CMRO2 and CBF in human PET studies. Our measurement system of CBF, CBV and oxygen metabolism is not only useful for studying neurovascular coupling, but also easily corroborates human PET studies.

Copyright © Neuroscience2014. All Right Reserved.