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Mood Disorders

開催日 2014/9/12
時間 14:00 - 15:00
会場 Poster / Exhibition(Event Hall B)

Contrasting expression pattern for immaturity and maturity marker genes in the dentate gyrus between mouse lines with immature dentate gyrus and mice overexpressing glucocorticoid receptor

  • P2-332
  • 小清水 久嗣 / Hisatsugu Koshimizu:1,2 萩原 英雄 / Hideo Hagihara:1,2 高雄 啓三 / Keizo Takao:2,3 宮川 剛 / Tsuyoshi Miyakawa:1,2,3 
  • 1:藤田保健衛生大学 / Div. of Sys. Med. Sci., ICMS, Fujita Hlth. Univ., Aichi, Japan 2:JST CREST / CREST, JST, Saitama, Japan 3:生理学研究所 行動・代謝分子解析センター / Ctr. for Gene. Anal. of Behav., NIPS, Aichi, Japan 

Adequate maturation of hippocampal neurons is thought to be crucial for normal cognitive function and emotional behavior, and dysregulation of cellular maturity in the hippocampus could be involved in mental disorders. Previously, we identified "immature dentate gyrus (iDG)" phenotype, in which almost all the granule cells in dentate gyrus (DG) exhibit pseudo-immature status, in several gene-targeted mouse lines that display behavioral abnormalities related to schizophrenia. On the other hand, the research group led by Huda Akil has reported that overexpression of glucocorticoid receptor (GR) in forebrain throughout the lifetime and in early life causes increased depression-like and/or anxiety-like behaviors in mice (Wei et al., PNAS, 2004; Biol. Psychiatry, 2012), raising the possibility that mice overexpressing GR (GRov mice) may represent a potential animal model for mood disorders, such as depression and anxiety disorder. In addition, overexpresson of GR caused an "aging-like" neuroendocrine phenotype and mild cognitive dysfunction in young mice (Wei et al., J. Neuroscience, 2007). In this study, we evaluated transcriptional maturation status of the DG of GRov mice using a bioinformatics tool, NextBio, with publicly available microarray data sets. The DG of GRov mice exhibit an opposite expression pattern of immaturity and maturity marker genes to the developing DG (P14 compared to P30) in wild-type (WT) mice, while gene expression pattern of those marker genes in iDG of Schnurri-2 knockout (Shn2 KO), alpha CaMKII heterozygous KO, and forebrain-specific calcineurin KO mice are similar to that in the developing DG (P14 compared to P30) of WT mice. Contrasting gene expression patterns were found between the DG of GRov mice and the mouse lines showing iDG phenotype. These observations indicate that maturation status of the DG in GRov mice may be changed potentially toward "over-maturity".

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