• Top page
  • Timetable
  • Per session
  • Per presentation
  • How to
  • Meeting Planner



Spinal cord Motor Neurous and Muscle

開催日 2014/9/11
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Role of RGMa inhibition in recovery of motor functions and axonal regeneration after spinal cord injury in macaques

  • P1-119
  • 中川 浩 / Hiroshi Nakagawa:1,2,3 二宮 太平 / Taihei Ninomiya:2,3 高田 昌彦 / Masahiko Takada:2,3 山下 俊英 / Toshihide Yamashita:1,3 
  • 1:京都大学霊長類研究所 / Dept Sys Neurosci, Primate Res Inst, Kyoto Univ, Aichi, Japan  2:大阪大学大学院医学系研究科 分子神経科学 / Dept Mol Neurosci, Osaka Univ, Osaka, Japan 3:Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) / Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) 

Impairments in dexterous finger movements after spinal cord injury (SCI) are often caused by disruption of the corticospinal tract (CST). Axonal plasticity and/or regeneration after SCI are considered to be critical to the recovery of motor functions. However, plastic changes in the adult mammalian central nervous system are prevented by the obstacles such as myelin, glial scar, and so on. The repulsive guidance molecule a (RGMa) was originally identified as an axon guidance molecule in the visual system. We previously reported using adult rats that RGMa expression was increased around the lesion site after SCI and, therefore, this molecule might be involved in suppression of axonal regeneration. In the present study, we showed the expression pattern of RGMa in the cervical cord after SCI in adult macaques. Moreover, we tested whether inhibition of RGMa could enhance the recovery of dexterous finger movements after SCI. We made a moderate extent of unilateral cervical cord injury at the C6/C7 border in rhesus monkeys. After the injury, RGMa was inhibited with anti-RGMa antibody. Multiple behavioral tests were then performed to assess the recovery of dexterous finger movements. Next, CST fibers were examined in the cervical cord by anterograde tract-tracing with biotinylated dextran amine. Immunohistochemical analysis revealed that RGMa was expressed in microglia/macrophages around the lesion site at day 10 after SCI, compared with a normal control. According to the behavioral assessment, the recovery of dexterous finger movements after SCI was largely enhanced in the anti-RGMa group, compared with the control group. The anterogradely labeled CST fibers were extended by application of the anti-RGMa antibody around the lesion site. The present results indicate that inhibition of RGMa promotes CST fiber extension around the lesion site after SCI and enhances the recovery of motor functions in macaques.

Copyright © Neuroscience2014. All Right Reserved.