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開催日 2014/9/13
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Temperature habituation is regulated by light and pheromone sensoryneuron in C. elegans

  • P3-173
  • 宇治澤 知代 / Tomoyo Ujisawa:1 太田 茜 / Akane Ohta:1 久原 篤 / Atsushi Kuhara:1 
  • 1:甲南大学大学院 / Grad. school of Sci., Konan Univ. 

Animals have adaptation mechanisms against environmental temperature changes. To understand its molecular mechanism, we are using simple model animal, nematode C. elegans. We recently found that C. elegans shows cultivation temperature-dependent cold tolerance, which is regulated by a single sensory neuron. After cultivation at 25 degree, C. elegans were destroyed by 2 degree cold stimuli. By contrast, most of animals can survive at 2 degree, after cultivation at 15 degree. Importantly, when 25 degree-cultivated animals are transferred to 15 degree and cultivated at 15 degree for only 3 hrs, animals can survive at 2 degree. This indicates that temperature memory for cold tolerance can be replaced for only 3 hrs. In order to determine the cells and genes underlying cultivation temperature-dependent cold tolerance, we utilized various mutants. We found that the mutant animals defective in TAX-4 cGMP-gated channel expressing in 10 pairs of sensoryneurons showed abnormal enhancement of cultivation temperature-dependent cold tolerance. The abnormality in tax-4 mutant is rescued by the specific expression of tax-4 cDNA in a single sensory neuron ASJ, which has been known as a photosensory neuron. In addition, we found that molecular components in photo-signaling pathway require for cold tolerance excluding LITE-1, photo receptor protein. Developmental defect in AFD known as thermosensory neuron did not affect the cold-tolerance. We are utilizing calcium imaging for WT and tax-4 mutant, and laser ablation at ASJ in WT to examine ASJ acts as a thermosensory neuron. The results suggest that the ASJ act as a thermosensory neuron, and control cold tolerance. To determine a molecular pathway for cultivation temperature-dependent cold tolerance, we referred previous DNA microarray analysis (Sugi et al., Nature neurosci., 2011). Then, DNA repair genes, endonuclease, are involved in the cold tolerance.

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