演題詳細
Oral
パーキンソン病とその類縁疾患 2
Parkinson's Disease and Related Disorders 2
| 開催日 | 2014/9/12 |
|---|---|
| 時間 | 18:10 - 19:10 |
| 会場 | Room I（311+312） |
| Chairperson(s) | 野元 正弘 / Masahiro Nomoto (愛媛大学大学院医学系研究科 薬物療法・神経内科学 / Department of Neurology and Clinical Pharmacologu, Ehime University, Graduate School of Medecine, Japan) 村田 美穂 / Miho Murata (国立精神・神経医療センター 病院・神経内科 / Department of Neurology, National Center Hospital, National Center of Neurology and Psychiatry, Japan) |
レボドパ誘発ジスキネジアモデルラットにおける側坐核のスパインの形態変化
Dendritic spines of medium spiny neurons in nucleus accumbens in 6-OHDA-lesioned rats chronically treated with levodopa
- O2-I-6-4
- 船水 章央 / Yukihisa Funamizu:1,3 上野 達哉 / Tatsuya Ueno:1,2 西嶌 春生 / Haruo Nishijima:1,2 上野 伸哉 / Shinya Ueno:2 八木橋 操六 / Soroku Yagihashi:3 冨山 誠彦 / Masahiko Tomiyama:1,2
- 1:青森県立中央病院 / Aomori Prefectural Central Hospital Department of Neurology, Japan 2:弘前大学医学研究科脳神経生理学講座 / Department of Neurophysiology Hirosaki University Graduate School of Medicine, Hirosaki, Japan 3:弘前大学医学研究科分子病態病理学講座 / Department of Pathology and Molecular Medicine, Hirosaki University Graduate School of Medicine, Hirosaki, Japan
Dendritic spines of medium spiny neurons in nucleus accumbens in 6-OHDA-lesioned rats chronically treated with levodopa
Yukihisa Funamizu1,3; Haruo Nishijima1,2; Tatsuya Ueno1,2; Shinya Ueno2; Soroku Yagihashi 3 ;Masahiko Tomiyama1,2
1Department of Neurology, Aomori Prefectural Central Hospital
2Department of Neurophysiology, and 3Department of Pathology and Molecular Medicine, Hirosaki University Graduate School of Medicine
Background:Dendritic spines of medium spiny neurons (MSN) in nucleus accumbens (NAc) in cocaine-treated rats increased in density and become enlarged. Although levodopa is most efficacious to ameliorate motor dysfunction in Parkinson's disease (PD), its chronic use to PD patients often induces dopamine dysregulation syndrome (DDS), a compulsive use of levodopa like addiction. The mechanisms of DDS have been suggested to be similar to those of cocaine addiction. We hypothesized that the morphological changes of dendritic spines, observed in cocaine-treated rats, also occur in NAc neurons in rats repeatedly treated with levodopa.
Objective:To examine morphological changes of dendritic spines in MSN in core and shell of NAc in 6-OHDA-lesioned rats repeatedly treated with levodopa.
Methods:We used control rats, 6-OHDA-lesioned rats (PD) and 6-OHDA-lesioned rats chronically treated with levodopa (levodopa-PD). After fixation, sections through NAc were prepared. Lucifer yellow was injected into soma of NAc MSN labeled by DAPI to visualize dendritic spines. We measured density and volume of spines using confocal laser scanning microscope and Neurolucida in MSN in the shell and core of NAc.
Results:Dopamine denervation decreased the density of spines in core and shell MSN, but levodopa treatment restored the decrement. Although spine volume was unchanged by dopamine denervation, levodopa treatment to the PD rats enlarged the dendritic spines.
Conclusions:The morphologic changes of dendritic spines in the levodopa-PD rats are like those observed in cocaine treated rats.