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演題詳細

Poster

感覚運動系の学習・可塑性
Sensorimotor Learning/Plasticity

開催日 2014/9/13
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

運動野損傷による前肢片麻痺ラットにおける巧緻運動の解析
Analysis of Skilled Forelimb Movement in Rats with Hemiplegia with Motor Cortex Lesion

  • P3-105
  • 間中 勇輝 / Yuuki Manaka:1 今村 一之 / kazuyuki imamura:1 
  • 1:前橋工科大学大学院 / Dept. Systems Life Engineering, Graduate School of Engineering, Maebashi Institute of Technology, Gunma, Japan 

We analyzed forelimb/paw and digits movement (skilled movement) in rats, performing a reaching task. Rats were placed in specially designed reaching box and trained to perform reaching of a single food pellet on a shelf. This task consist of components of i) rat approaching the front of the box, ii) reaching to a single pellet through the slot and iii) retrieving it with its preferred hand, iv) returning to the back of the box. Using high-speed video recording from two orthogonal directions and frame-by-frame video analysis, we quantitatively elucidated how forelimb/paw and digits moved during the reaching task. Following three points were analyzed. (i) distance between rat's second digit-tip and fifth digit-tip of the hand, (ii) the angle between the horizontal plane and straight line comprised of the two digit-tip, (iii) positional relationship between each digit-tip and a pellet. Analyses indicated that forepaw displayed maximum opening when the forepaw was pronated over the pellet. The range of angle was 0-60 degrees during pronation and 0-130 degrees during supination. Reaching to the pellet indicated high precision and the precision was order of 1 mm. Then, experimental lesion was made to the forelimb motor cortex contralateral to the preferred forelimb. After recovery from the surgery, similar analyses of the forearm movement was conducted and compared to the data of pre-lesion. The results indicated that the speed of hand opening and closing was decreased. Before the lesion, time of opening and closing was measured 0.1 seconds. In contrast, the time was extended 0.2 seconds after the lesion. The misdirection of each digit-tip was also found after the lesion of motor cortex. In conclusion, we succeeded in quantitative analysis of skilled movement of rats, performing a reaching task, by developing original software. These findings suggest some important clues in rehabilitation of hand hemiplegia and skilled movement impaired by stroke to motor cortex.

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