演題詳細
Oral
生殖・摂食・代謝調節
Reproduction, Feeding and Metabolic Regulation
| 開催日 | 2014/9/12 |
|---|---|
| 時間 | 10:00 - 11:00 |
| 会場 | Room J（313+314） |
| Chairperson(s) | 功刀 浩 / Hiroshi Kunugi (独立行政法人国立精神・神経医療研究センター 神経研究所 / Department of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Japan) 上田 陽一 / Yoichi Ueta (産業医科大学医学部 第１生理学 / Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Japan) |
GnRHニューロンにおけるカルシウム透過性AMPA受容体へのシナプス取り込みがLHサージの発生に影響する
Phasic synaptic incorporation of GluR2-lacking AMPA receptors at gonadotropin-releasing hormone neurons is involved in the generation of the luteinizing hormone surge in female rats
- O2-J-2-1
- 黒木 洋子 / Yoko Kuroki:1 多田 敬典 / Hirobumi Tada:1 船橋 利也 / Toshiya Funabashi:1,2 紙谷 義孝 / Yoshitaka Kamiya:3 後藤 隆久 / Takahisa Goto:5 須山 紅美子 / Kumiko Suyama:1 佐野 亜加根 / Akane Sano:1 美津島 大 / Dai Mitsushima:1 Etgen Anne M / Anne M Etgen:4 高橋 琢哉 / Takuya Takahashi:1,4
- 1:横浜市立大学大学院・医・生理学 / Dept. of Physiology Yokohama City University Graduate School of Medicine, Kanagawa, Japan 2:聖マリアンナ医大医生理 / Dept Physiol, St.Marianna University School of Medicine, Kanagawa, Japan 3:新潟大学医麻酔科 / Dept Anesthesiology, Niigata University Graduate School of Medicine, Niigata, Japan 4:Dept Neuroscience,Albert Einstein College of Medicine, NY, USA / Dept Neuroscience,Albert Einstein College of Medicine, NY, USA 5:横浜市立大学医麻酔科 / Dept Anesthesiology, Yokohama City University Graduate School of Medicine, Kanagawa, Japan
Reproductive success depends on a robust and appropriately timed preovulatory luteinizing hormone (LH) surge, which is induced by the activation of gonadotropin-releasing hormone (GnRH) neurons in response to positive feedback from increasing estrogen levels. However, the neuronal and molecular basis for the generation of the GnRH/LH surge is still unclear. Here we document an increase in postsynaptic GluR2-lacking Ca2+-permeable AMPA-type glutamate receptors (CP-AMPARs) at synapses on GnRH neurons on the day of proestrus in rats, coincident with the increase in estrogen levels. Functional blockade of CP-AMPARs depressed the synaptic responses only on the day of proestrus and concomitantly attenuated the LH surge. Thus, the phasic synaptic incorporation of postsynaptic CP-AMPARs on GnRH neurons is involved in the generation of the LH surge. Our results represent the first evidence of a phasic change in the stoichiometry of synaptic AMPARs, and they delineate its physiological role.