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演題詳細

Poster

成体ニューロン新生
Adult Neurogenesis

開催日 2014/9/13
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

睡眠剥奪による海馬神経新生の減少は特に腹側海馬で強く見られる
Sleep deprivation decreases adult neurogenesis, dominantly in ventral hippocampus

  • P3-097
  • 岡 綾奈 / Ayana Oka:1 村田 雄介 / Yusuke Murata:1 井石 斐耶 / Ayaka Iseki:1 森 征慶 / Masayoshi Mori:1 美根 和典 / Kazunori Mine:2 遠城寺 宗近 / Munechika Enjoji:1 
  • 1:福岡大薬・臨床薬物治療 / Dept Pharmacother, Fac Pharmaceuti Sci, Fukuoka Univ, Fukuoka, Japan 2:医療法人社団水戸病院神経内科精神科 / Faculty of Neurology and Psychiatry, Mito Hospital 

[Introduction]
Insomnia is not only a major symptom, but a risk factor of depression. However, the exact mechanism that insomnia contributes to the onset of depression remains unclear. Several authors reported that sleep deprivation over 3 days resulted in the reduction of rat hippocampal neurogenesis. Hippocampus is functionally subdivided along the septotemporal axis, the dorsal part is related to learning and memory and the ventral part regulates emotion. We hypothesize that the impact of sleep deprivation may be different in dorsal and ventral hippocampus. In this study, we evaluated the regional differences in the effects of sleep deprivation on adult hippocampal neurogenesis.
[Methods]
Sprague-Dawley male rats (7 weeks age) were used in all experiments. After 1 week habituation session, animals were exposed to sleep deprivation for 1 or 3 days using the platform-over-water method. Immediately after sleep deprivation session, rats were perfused with 4% paraformaldehyde and brains were cut into 40-μm coronal sections by using freezing microtome. A series of sections were used in immunostaining for doublecortin (DCX), a marker of newly generated neurons, and the number of DCX-positive cells in the dentate gyrus of hippocampus was quantified in dorsal and ventral hippocampus.
[Results]
The number of DCX-positive cells was significantly decreased in sleep deprivation for 3 days group compared with sham for 3 days group both in dorsal and ventral hippocampus. (dorsal, -15.7%; ventral,-27.5%)
No significant differences were observed between sleep deprivation and sham for 1 day groups.
[Discussion]
In this study, sleep deprivation resulted in the decrease in hippocampal neurogenesis dominantly in ventral part. Our results suggest that the reduction of hippocampal neurogenesis induced by sleep loss may contribute to the onset of depression. In further study, we need to evaluate the temporal change of neurogenesis after sleep deprivation session.

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