Establishing a reliable test to assess behavioral inhibition in animal models is essential to elucidate the mechanisms of impulsive behaviors seen in patients with psychiatric disorders, such as attention deficit hyperactivity disorder (ADHD). In the present study, the Variable Time Wait Task (VTWT) and Go/No-go (GNG) tasks using a touchscreen device were developed to assess behavioral inhibition in mice. 10-week old male C57Bl/6J (B6) mice and DBA/2J (D2) mice were tested in a chamber equipped with a touchscreen monitor. In the VTWT, touching the start cue (white cross) initiated the trial, and a reward cue (white panel) was displayed after a random variable blank period (1-3 sec, 3-5 sec and 1-5 sec). In the GNG task, each mouse was assigned a Go or a No-go stimulus. The stimulus consisted from either of the visual cues; black and white stripes with upward motion and a still white windmill on a black background. Each trial was initiated with a start cue, and the Go or No-go stimulus was presented after a blank period. In order to obtain a reward, the mice had to touch the monitor in response to a Go stimulus and withhold touching it during the No-go stimulus to obtain a reward. The result of VTWT showed that both B6 and D2 groups were able to wait for maximum duration of 5 sec with an average final correct rate of 70%. Moreover, the intra-individual variability of response latency to reward cue was significantly increased in the D2 group. In the GNG task, B6 and D2 groups had scores from 0-10% (Session 1) to 70% (Final session) in the No-go correct response rate. The Go correct response rate remained constant between 90 to 100% in both groups throughout the sessions. In conclusion, the test we have developed to assess the inhibitory behavioral control can successfully evaluate the inhibition and sustained attention in mice.