The nicotine exposure or cigarette smoking during pregnancy is associated with cognitive disabilities in the offspring, such as attention deficit hyperactivity disorder. Using animal models of prenatal nicotine exposure have supported the hypothesis that nicotine may directly and/or indirectly cause impairments in fetal and neonatal development and can be an animal model of hyperactivity disorder. Therefore we examined the instinctive behavioural changes in prenatal nicotine exposure male mice to confirm the establishment of animal model for hyperactivity disorders. Male mice with or without utero nicotine exposure were used in 3, 6, 9 and 12 week olds. Using the behavioral indices of open field, elevated plus maze, hot plate and novel object recognition tests as the behavioral examinations was done in this study. The data showed that the experimental group compared to the control one had the higher locomotor activity in open field test, no difference in open arm of elevated plus maze test, lower trend in duration of hot plate test and similar performance in the novel object recognition test. The male mice with prenatal nicotine exposure can be an animal model for hyperactivity disorders and be valuable as the screen tools for evaluating the therapies for hyperactivity disorders.