Hypobaric hypoxia (HH) is known to cause cognitive dysfunctions owing to the high oxygen dependency of the brain. Cognitive and motor deficits have been reported to occur on chronic exposure to HH. On the other hand various studies have shown that enriched environment enhances learning and memory, reduces memory decline in aged animals, decreases anxiety and increases exploratory activity and also increases dendritic branching and length, the number of dendritic spines and the size of synapses on some neuronal. Previously we have shown that enriched environment improves cognition and decreases neurodegeneration during chronic exposure to hypobaric hypoxia. Present study is designed to explore the effect of enriched environment on hypobaric hypoxia induced alteration in synaptic plasticity and strength. Sprague dawley rats (3 months old) were exposed to hypobaric hypoxia condition in an animal decompression chamber at an altitude of 25000 feet for continuous 7 days. Animals from control group were kept in standard cage whereas enriched group were kept in enriched environment cage. Following exposure rats were sacrificed and hippocampi was dissected out and processed for different experimental protocols. MALDI following 2D electrophoresis was performed to study the global change in hippocampal protein expression. Further expression of different synaptic proteins was assessed through western blotting and immunohistochemistry. Golgi stain was performed to study dendritic arbrorization. Results of the present study showed that identified proteins belong to a diverse variety of functional classes including cell death, proteins involved in oxidative stress metabolism, synaptic proteins, growth factors and proteins associated with signalling. Hypobaric hypoxia decreases expression of synaptic proteins i.e. synaptophysin, PSD-95, synaptotagmin which further validated by western blotting whereas exposing them in enriched environment ameliorate this synaptic loss. Golgi staining reveals protective effect of enriched environment during HH exposure as evident from increased dendritic arborisation, spine density and morphology.