• Top page
  • Timetable
  • Per session
  • Per presentation
  • How to
  • Meeting Planner



Spatiotemporal regulation of glutamate distribution by transporters (EAATs/VGLUTs) and implications in neuropsychiatric disorders

開催日 2014/9/13
時間 9:00 - 11:00
会場 Room E(301)
Chairperson(s) 田中 光一 / Kohichi Tanaka (東京医科歯科大学難治疾患研究所分子神経科学分野 / Medical Research Institute & CBIR, Tokyo Medical & Dental University, Japan)
木下 専 / Makoto Kinoshita (名古屋大学大学院理学研究科生命理学専攻 / Department of Molecular Biology, Nagoya University Graduate School of Science, Japan)

The trimeric glutamate transporter core domain is the astrocyte process tip localizer / stabilizer

  • S3-E-1-6
  • 林 真理子 / Mariko Kato Hayashi:1 安井 正人 / Masato Yasui:1 
  • 1:慶應義塾大学 / Keio University, Japan 

Following synaptic activation, excitatory neurotransmitter glutamate released to synapses must be removed by glutamate transporters to terminate the signal and to protect neurons from excitotoxicity. Although neurons also contribute, most of the released glutamate is taken up by astrocytes. For this purpose, astrocytes extend thousands of thin cellular processes to surround synapses within neuronal networks. The mechanisms underlying the localization of glutamate transporters within these finely branched processes, however, are not known. In this study, we found that the trimeric transmembrane transporter domain of glutamate transporters localizes to the tips of filopodia, even after deletion of their N- and C-terminal cytoplasmic tails, which contain binding sites for cellular proteins. Furthermore, the trimeric transporter domain of the neutral amino acid transporter ASCT1 also localized to the filopodia tips, suggesting that filopodia tip localization is a property common to trimeric transporters. Neither transporter activity nor astrocyte-specific proteins are required for filopodia tip localization. We also found that transporter cores localized to filopodia tips strengthen the attachment of the filopodia to external substrates, thereby stabilizing the filopodia. The trimeric core domains of glutamate transporters also localized to finely branched astrocyte processes, analogous to filopodia, in organotypic hippocampal slice cultures. Together, our results demonstrate that, in addition to glutamate uptake, glutamate transporters play a structural role in promoting the stabilization of astrocyte fine processes.

Copyright © Neuroscience2014. All Right Reserved.