Distribution of Pacinian corpuscles (PCs) in human fingers was investigated in detail by Cauna and Mannan (1958). However, it has not been viewed three dimensionally (3D) until now. We investigated three dimensional distribution of PCs and their afferents in a infant toe. A distal part of a toe excised from a polydactyl patient (1year-old; informed consented by parents and obtained the permission of Hospital Ethics Committee of Kyoto Prefect. Univ. Med.). The specimen of the toe contained distal phalange and distal half of middle phalange (10 mm wide, 11mm high and nail width was 5.5mm) was immersed in 4% paraformaldehyde in 0.1 M phosphate buffer. After decalcification in 10 % EDTA (pH 7.4), the specimen was cut into 100 μm-thick serial sections on a cryostat. All serial sections were immunohistochemically stained using primary antibodies against protein gene product 9.5 (PGP9.5) and myelin basic protein to reveal axons and myelin sheath, and fluorescence (Alexa 468 or 568) conjugated secondary antibodies appropriately. PCs were recognized by PGP9.5-positive axon terminals with lamellae under light microscope. All PCs were mapped on each sectional image and reconstructed by software. Totally 47 PCs existed and most of them were distributed in ventral side of the toe. All corpuscles distributed in the subcutaneous tissue (42 PCs) or beneath tendons (5 PCs) but not in the dermis. Most PCs were solitarily scattered, but 2~5 PCs grouped together in 12 parts. No PCs were observed between the distal phalange and the nail. Most PCs were distributed on the periphery part of the phalanges. Transverse diameter of PCs was 0.1mm~0.5mm and longitudinal was 0.5mm~1.4mm. Their long axes were all parallel to the skin surface, but their directions were variable. When axons of 5 PCs in 2 groups were traced proximally, no afferents were merged into one as far as observed in nerve bundles in the subcutis. These results may suggest that PCs are possibly more sensitive to the pressure of skin and movement of the joint and tendon rather than that of bones and nail themselves.