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Signal Transduction and Modulation

開催日 2014/9/12
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Enhanced CICR and SOCE in Layer 3 Pyramidal Cells in the Barrel Cortex of PRIP-DKO Mice

  • P2-051
  • 河野 奨 / Tsutomu Kawano:1 豊田 博紀 / Hiroki Toyoda:1 齋藤 充 / Mitsuru Saito:1 佐藤 元 / Hajime Sato:1 兼松 隆 / Takashi Kanematsu:2 平田 雅人 / Masato Hirata:3 姜 英男 / Youngnam Kang:1 
  • 1:大阪大院・歯・高次脳口腔機能 / Dept. Neurosci. & Oral Physiol., Osaka Univ. Grad. Sch. Dent., Osaka, Japan 2:広島大院・医歯薬保・細胞分子薬理 / Dept. Dent. Pharmacol., Grad. Sch. Biomed. Scis., Hiroshima Univ., Hiroshima, Japan 3:九州大院・歯・口腔細胞工学 / Lab. Mol. Cell. Biochem., Fac. Dent. Sci., Kyushu Univ., Fukuoka, Japan 

The two subtypes of phospholipase C-related but catalytically inactive protein (PRIP-1/2) were first identified as novel IP3-binding proteins. IP3-induced Ca2+ release (IICR) was impaired in cultured cortical neurons from PRIP-1 KO mice while store-operated Ca2+ entry (SOCE) was enhanced in hematopoietic B cells in PRIP-2 KO mice. In the present study, we aimed to clarify whether and how Ca2+ release from intracellular Ca2+ stores and SOCE are different between layer 3 (L3) pyramidal cells (PCs) in PRIP-1/2-DKO and WT mice. The amplitude of Ca2+ transients was measured as a difference in the F340/F380 ratio from the baseline level in L3 PCs in slice preparations incubated with fura-2 AM. A mixed solution of 20 mM K+ and 20 mM caffeine induced large Ca2+ transients known as Ca2+-induced Ca2+-release (CICR), which decayed to a plateau level that remained almost constant at least for 10 min examined. The mean peak and plateau amplitudes of Ca2+ transients in the PRIP-DKO PCs were significantly larger than those in the WT PCs. Next, whether SOCE occurred following CICR or not was directly examined by reducing [Ca2+]o. The CICR was induced twice consecutively by the first and second applications of the caffeine/high-K+ solution, which were separated by 35-40 min. Immediately after the first and second CICR, the caffeine/high-K+ solution was changed to the Ca2+-free extracellular solution and the standard extracellular solution containing 2 mM Ca2+, respectively.

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