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演題詳細

Poster

その他の応用
Other Applications

開催日 2014/9/13
時間 14:00 - 15:00
会場 Poster / Exhibition(Event Hall B)

オプトジェネティックスを用いた触覚パターンによるバレル野のfMRI応答
fMRI responses to optogenetic tactile pattern of whiskers in barrel cortex of channelrhodopsin-2 expressing rat

  • P3-376
  • 横山 超一 / Yukinobu Yokoyama:1,2 住吉 晃 / Akira Sumiyoshi:3 川島 隆太 / Ryuta Kawashima:3 八尾 寛 / Hiromu Yawo:1,2 
  • 1:東北大学大学院 生命科学研究科 脳機能解析分野 / Tohoku University Graduate School of Life Sciences Laboratory of Molecular and Cellular Neurosciences 2:戦略的創造研究推進事業 / CREST, JST 3:東北大学 加齢医学研究所 / Tohoku Univ. IDAC, Sendai, Japan 

The rodent whisker-barrel cortical system has been a model to reveal somatosensory representation in the brain. Optogenetics would facilitate this with high spatiotemporal resolutions. Recently, we have identified the expression of ChR2 in the mechanoreceptive neurons in the trigeminal ganglion (TG) in one of thy1.2-channelrhodopsin 2 (ChR2)-Venus transgenic rat lines, W-TChR2V4 (Honjoh et al., 2014). Each whisker follicles were also richly innervated by the ChR2-positive nerve endings.
The whiskers were all trimmed and 16 follicles were attached with plastic optic fibers in array of 4x4. Each endings of optic fiber was connected to blue LED, which turned on and off independently by a pulse generated by a computer. Therefore, the whiskers could be stimulated with various spatiotemporal patterns (optogenetic tactile pattern, OTP). The functional magnetic resonance imaging (fMRI) responses of barrel cortex were investigated using OTP under 7T-MRI system.
The whisker photostimulation induced a change of blood oxygenation level-dependent (BOLD) responses in the barrel field of contralateral somatosensory cortex in a manner dependent on time. The response to a single whisker phostimulation was more regional than the simultaneous 16-whisker photostimulation.
It is suggested that the OTP was accompanied with specific spatiotemporal changes of fMRI response. Our OTP, in combination with fMRI, would facilitate to study how the spatiotemporal pattern of the whisker mechanoreception would be represented in the cortex.
All animal procedures were conducted in accordance with the guiding principles of Physiological Society of Japan and NIH.

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