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開催日 2017/7/22
時間 15:00 - 16:00
会場 Poster (Hall 8, International Exhibition Hall)
  • 3P-377   Time: 15:00 - 16:00
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シリコンウィスカ電極を用いたマウス及びサル大脳皮質単一ニューロン計測
Title: Single unit recordings from mouse and monkey cortices using the microscale silicon whisker electrode devices

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  • 澤畑 博人 / Hirohito Sawahata:1 山際 翔太 / Shota Yamagiwa:1 大井 英生 / Hideo Oi:2 沼野 利佳 / Rika Numano:3 石田 誠 / Makoto Ishida:1,2 鯉田 孝和 / Kowa Koida:4 河野 剛士 / Takeshi Kawano:1  : 
  • 1:豊橋技術科学大学 電気・電子情報工学系 / Dept Electrical and Electronic Info Eng, Toyohashi Univ of Tech, Aichi, Japan 2:豊橋技術科学大学 エレクトロニクス先端融合研究所(EIIRIS) / EIIRIS, Toyohashi Univ of Tech, Aichi, Japan 3:豊橋技術科学大学 環境・生命工学 / Dept Computer Sci and Eng, Toyohashi Univ of Tech, Aichi, Japan 4:豊橋技術科学大学 情報・知能工学 / Dept Environmental and Life Sci, Toyohashi Univ of Tech, Aichi, Japan 

We have proposed a fine needle-electrode (<10 μm in diameter) fabricated by selective vapor-liquid-solid (VLS) growth of silicon `whisker' needle (Fujishiro et al., 2014; Sawahata et al., 2016). Such fine electrodes potentially offer high density arrangement of the needles with a low invasive penetration in vivo. However, due to the needle length of previous electrodes (<200 μm), neuronal recordings were limited for superficial layers of the corticies. Thus difficuluties remain for recording from deeper layers of the mouse corticies and from large animals such as primates. Here, we solved this geometrical issue by extending the needle length in the VLS growth. Fabrication processes were same as previous ones (Sawahata et al., 2016), excepting for the duration of the VLS growth. Although it is generally difficult to grow the needles longer than 200 μm because of inhomogeneous growth temperature in the needles, the growth duration for ~7 hours results in 400-μm-length Si needles. Diameter of the needle was <3 μm. The needle tip was covered with platinum (Pt) and the needle sidewall was insulated by parylene-C. After the device packaging, platinum-black (Pt-black) was deposited on the needle tip for reduction of the needle impedance (diameter of Pt-black tip was 5-μm). As the results, the impedance measured in saline was about 328 kΩ ± 221 kΩ (mean ± SD, n = 3) at 1 kHz, which is low enough to record neuronal signals. After the electrical characterizations, we demonstrated in vivo neuronal recordings from the primary visual cortex (V1) and the primary somatosensory cortex (S1) of anesthetized mice and from the V1 of an awake monkey. As a result, single unit signals were successfully recorded, while the sensory responses were clearly observed. These results indicate that the fabricated 5-μm-diameter and 400-μm-length needle-electrode can be used for the single unit recording from the cortical neuron of various mammals under low invasion.

 

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