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Deciphering neural activity using novel genetically encoded voltage and calcium indicators


開催日 2017/7/20
時間 08:45 - 10:45
会場 Room 2 (Convention Hall B, 2F, International Conference Hall)
Chairperson Micheal Z Lin / Micheal Z Lin ( Stanford University School of Medicine / Stanford University School of Medicine )
  • 1S02m-5   Time: 10:10 - 10:30

Fast two-photon imaging of electrical activity with ASAP-family voltage indicators

  • Michael Z Lin:1 Mariya Chavarha:2 Stephen W Evans:1 
  • 1:Department of Neurobiology, Stanford University 2:Department of Bioengineering, Stanford University 

Genetically encoded fluorescence indicators of transmembrane voltage with suitable speed and responsivity to report neuronal excitation have long been desired. Optical voltage tracking would enable reporting of activity from multiple neurons, from specific neuronal subpopulations, or from neurons in vivo over time. Compared to genetically encoded calcium indicators, genetically encoded voltage indicators have higher temporal precision and can detect subthreshold responses as well. We have been developing ASAP-family voltage indicators based on GFP and a classical voltage-sensing domain. In recent work, we have developed new variants ASAP2s and ASAP3 with improved responsivity. As with earlier ASAPs, the new variants respond similarly under two-photon and one-photon illumination. In combination with rapid two-photon scanning methods, ASAP2s and ASAP3 enable detection of action potentials with sub-millisecond kinetics in brain slices and mice.


研究助成:Research funds : NIH grant 1U01NS090600-01

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