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Axonal/Dendritic Growth and Circuit Formation

開催日 2014/9/12
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Exploration of genes regulating dendritic spine pruning in the cerebral cortex of the common marmoset

  • P2-095
  • 佐々木 哲也 / Tetsuya Sasaki:1 小賀 智文 / Tomofumi Oga:1,2 中垣 慶子 / Keiko Nakagaki:1 境 和久 / Kazuhisa Sakai:1 住田 佳代 / Kayo Sumida:3 星野 耕平 / Kohei Hoshino:4 宮脇 出 / Izuru Miyawaki:4 斎藤 幸一 / Koichi Saito:3 須藤 文和 / Fumikazu Suto:1 一戸 紀孝 / Noritaka Ichinohe:1 
  • 1:国立・精神神経セ・神経研・微細構造 / Dept of Ultrastructural Study, Nat Inst of Neurosci, NCNP, Tokyo, Japan 2:大阪大院・生命機能・認知脳科学 / Lab of Cognitive Neurosci, Grad Sch Frontier Biosci, Osaka Univ, Osaka, Japan 3:住友化学・生物環境科学研・細胞科学 / Environmental Health Sci Lab, Sumitomo Chemical Co., Ltd., Osaka, Japan 4:大日本住友製薬・前臨床研・安全性第1 / Preclinical Research Lab, Dainippon Sumitomo Pharma Co., Ltd., Osaka, Japan 

In the primate cerebral cortex, dendritic spines rapidly increase in number after birth up to infantile or mid-childhood, and then decrease towards adulthood. Abnormalities in these processes, especially in the pruning phase, accompany several psychiatric disorders. We have been investigating the normal processes of spinogenesis/pruning, and the molecular mechanisms underlying them, in the cerebral cortex of the common marmoset. Our previous study showed that, in the primary visual area, an inferior temporal area, and a prefrontal cortical area 12, the total spine number in basal dendrites of a layer-III pyramidal cell reached a peak at 3 postnatal months (3M) and then markedly declined (Oga et al., 2013). In this study, we investigated gene expression in the same areas at newborn, 2M, 3M, 6M, and adult using marmoset DNA microarray with 23,016 probes. As a step toward clarifying the molecular basis of spine pruning, we compared gene expression at 3M (peak of spinogenesis) and 6M (pruning period). Based on the criterion of at least 1.2 fold change, we found that 1,577 genes showed differential expression between 3M and 6M in the 3 areas examined. Canonical pathway analysis revealed that a large number of these genes belong to subsystems within the axon guidance signaling, immune system, glutamate receptor signaling, and G-protein-coupled neuromodulator system. Among them, axon guidance signaling and immune system showed high plausibility (low p value). Expression patterns of the selected genes were confirmed by in situ hybridization. These results suggest that molecular components within these signaling pathways are potential candidates regulating pruning phase in the primate cortex.

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