Many engineers have created communication systems that enhance a sense of presence or reality to realize natural communications with those in remote locations. However, there are very few methods by which a sense of presence or reality can be quantitatively evaluated. Here we describe a quantitative evaluation of the acoustic sensation of presence by measuring autonomic responses. We examined the effects of acoustic information about speakers' movements on the sense of presence in personal communication by using a three-dimensional sound field reproduction system based on the boundary surface control principle, by which listeners can experience high realistic sensation of speakers. We prepared two types of speech stimuli, "dynamic" and "static". In a dynamic condition, the speakers' speech was recorded along with their subtle unconscious movements. In a static condition, the speech stimulus in a dynamic condition presented from a mouth simulator was recorded to remove any information about the speakers'movements. The sense of the speakers' presence and friendliness was assessed subjectively by the participants. In physiological experiments, we evaluated the autonomic responses by measuring the blood volume pulse amplitude and the skin conductance response during the speakers' voice presentation. We found that a higher sense of presence was observed in the dynamic condition than in the static condition, and that the participants expressed a higher friendliness for speakers in the dynamic condition. Moreover there were differences between the autonomic nervous system activities in the dynamic and the static conditions. These findings suggest that a sense of presence is influenced by acoustic information about speakers' unconscious subtle movements and that the existence or non-existence of speakers' movements can be detected from the autonomic responses.