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Visual System

開催日 2014/9/11
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

マウス一次視覚野におけるDiacylglycerol lipase-αの発達期可塑性への寄与
Contribution of diacylglycerol lipase-α to developmental plasticity in mouse V1

  • P1-177
  • 亀山 克朗 / Katsuro Kameyama:1 米田 泰輔 / Taisuke Yoneda:1 後藤 隆浩 / Takahiro Gotou:1 寺田 慧子 / Keiko Terata:1 中西 真実 / Mami Nakanishi:1 崎村 建司 / Kenji Sakimura:2 狩野 方伸 / Masanobu Kano:3 畠 義郎 / Yoshio Hata:1 
  • 1:鳥取大院・医・生体高次機能 / Div Integrative Biosci, Tottori Univ Grad Sch Med Sci, Yonago, Japan 2:新潟大・脳研・細胞神経生物学 / Dept Cellular Neurobiol, Brain Res Inst, Niigata Univ, Niigata, Japan 3:東京大院・医・神経生理 / Dept Neurophysiol, Tokyo Univ Grad Sch Med, Tokyo, Japan 

Ocular dominance plasticity (ODP) is a well-documented model of the experience dependent development of neural circuit. Growing evidences suggest that the layer specific contribution of cannabinoid receptor type 1 (CB1) in ODP of V1. Previously, we showed that CB1 mainly exists in layer II/III and VI of V1 and its expression is regulated in development- and visual input-dependent manner in mouse V1 (Yoneda et al., 2013). However, it is still unknown which endocannabinoids (eCB) contribute to visual cortical plasticity. Because 2-arachidonoylglycerol (2-AG) is supposed to be a major eCB that is synthesized by diacylglycerol lipase-α (DGL-α) at postsynaptic sites, we examined the expression of DGL-α in developing mouse V1 and the possible role of DGL-α in ODP.
Histological analysis uncovered that DGL-α is strongly expressed at lower part of layer II/III and IV in V1. Interestingly, the distribution of CB1 and DGL-α appears complementary along cortical layers. The relative amount of DGL-α protein in V1 significantly increased during development and reached the maximum level around the critical period of ODP. The amount of DGL-α protein decreased in mice reared in darkness from birth to P30 but not in those reared in darkness to P50.
Pharmacological inhibition of DGL by cortical application of tetrahydrolipstatin (THL) blocked ODP in mouse V1 at the critical period. ODP was inhibited in DGL-α knockout mice, but not in DGL-β knockout mice. DGL-α dependent ODP was more prominent in the layer II/III than the other layers. The binocular matching of preferred orientation of V1 neurons was also disrupted in DGL-α knockout mice.
These results suggest that the activity of DGL-α is necessary for the developmental plasticity of V1, and visual input influences the postnatal development of DGL-α expression.

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