演題詳細
Poster
学習・長期記憶
Learning and Long-term Memory
開催日 | 2014/9/12 |
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時間 | 11:00 - 12:00 |
会場 | Poster / Exhibition(Event Hall B) |
キノコ体特異的に発現するsmall GTPase Rgk1はショウジョウバエ嗅覚学習記憶の麻酔耐性記憶に特異的に働く
A mushroom-body-specific small GTPase Rgk1 regulates the anesthesia-resistant component of Drosophila olfactory memory
- P2-239
- 村上 智史 / Satoshi Murakami:1 南 真樹 / Maki Minami:1 中戸 隆一郎 / Ryuichiro Nakato:2 白髭 克彦 / Katsuhiko Shirahige:2 多羽田 哲也 / tetsuya tabata:1
- 1:東京大学分子細胞生物学研究所神経生物 / ILab of Neuroscience, IMCB, Univ. of Tokyo. 2:東京大学分子細胞生物学研究所ゲノム情報解析 / Lab of Genome structure and function, IMCB, Univ. of Tokyo.
Animals adapt to ever-changing environments by making novel associations of stimuli and altering innately engrained behaviors accordingly. On making and storing such novel associations, animals form memory consisting of several components with different durations. One example is a Drosophila olfactory aversive learning in which the anesthesia-sensitive memory (ASM) and the anesthesia-resistant memory (ARM) are concurrently generated in a single conditioning session. While several studies suggests the distinction between ASM and ARM in neuronal or molecular level, underlying molecular mechanism is still obscure, mainly due to a lack of information on genes specifically act in each memory component. In the present study we identified a novel gene rgk1 as a specific regulator of ARM formation. Rgk1 belongs to a conserved Ras-related small G-protein RGK family whose functions include the regulation of the Ca2+ channel and the neurite remodeling. We isolated rgk1 in a screening for genes expressed in the mushroom body (MB), a cardinal neuronal structure of olfactory associative memory. Subsequent antibody and knockdown studies demonstrated specific requirement of MB-expressing Rgk1 for ARM, and an experiment with rgk1-miRNA localized the rgk1 function in a subset of MB neurons. A genetic interaction suggests rgk1 may act closely with dunce (a gene encoding a phosphodiesterase), raising a possibility Rgk1 regulating ARM through the modulation of the cAMP pathway. Importantly, over-expression of rgk1 led to an enhanced memory that mainly consists of ARM, pointing to an instructive role of Rgk1 in ARM formation. Thus, our findings demonstrate an importance of Rgk1 in understanding molecular mechanisms that determine the duration of memory whereby animals maximize their adaptation to an environment.