• Top page
  • Timetable
  • Per session
  • Per presentation
  • How to
  • Meeting Planner



Learning and Long-term Memory

開催日 2014/9/12
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

A study on the role of CB1 receptors in motor learning using the three-lever operant task

  • P2-251
  • 米田 貢 / Mitsugu Yoneda:1 田端 佑樹 / Yuki Tabata:1 高林 亮 / Ryo Takabayashi:1 少作 隆子 / Takako Ohno-Shosaku:1 
  • 1:金沢大学・医薬保健研究・保健学系・リハビリ / Fac Health Sci, Kanazawa Univ, Kanazawa, Japan 

Endocannabinoids play an important role in synaptic plasticity at various types of synapses throughout the brain, by acting on CB1 receptors. In the basal ganglia, which are involved in motor learning, CB1 receptors are expressed on input and output terminals, and involved in endocannabinoid-mediated synaptic plasticity. Therefore, it is likely that CB1 receptors play a role in motor learning. To test this possibility, we used the three-lever operant task, which we established to elucidate the neural basis of motor sequence and skill learning, originally for rats and later for mice. In this task, animals were trained to press three levers in a given sequence. We previously reported that the performance of this task was impaired in Parkinson's model rats, indicating its dependence on the basal ganglia. In the operant box used for mice, three levers (1.8 × 1.5 cm) were protruded 1.8 cm into the chamber, and the right(A), center(B) and left(C) levers were positioned 2, 4 and 2 cm above the floor, respectively. One training session lasting 60 min was given once a day and five times a week. At the age of eight weeks, mice were trained to press any one of the three levers for a food reward as shaping, and then trained to press three levers in a given sequence with or without time limitation. In the task with time limitation, mice were required to press the second (or third) lever within a given time (T) after the onset of the first (or second) lever press. Wild-type mice showed good performance (more than 100 pellets in one session) at T= 0.5 - 0.8 s. When the order was reversed from ABC to CBA, wild-type mice learned the new order within 5 sessions. CB1-knockout mice displayed impaired performance in the three-lever operant task. The present study suggests that the three-lever operant task is useful for examining the role of CB1 receptors in motor sequence and skill learning in mice.

Copyright © Neuroscience2014. All Right Reserved.