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Neuroprotection, Neurotoxicity and Neuroinflammation

開催日 2014/9/11
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Estimation of immunogenicity of transplantation therapy with iPS cells by PET imaging

  • P1-337
  • 高良 沙幸 / Sayuki Takara:1 水間 広 / Hiroshi Mizuma:1 林 拓也 / Takuya Hayashi:1 土居 久志 / Hisashi Doi:1 武田 千穂 / Chiho Takeda:1 馬渡 彩 / Aya Mawatari:1 辻 美恵子 / Mieko Tsuji:1 土井 大輔 / Daisuke Doi:2 森実 飛鳥 / Asuka Mirizane:2 菊池 哲広 / Tetsuhiro Kikuchi:2 高橋 淳 / Jun Takahashi:2 尾上 浩隆 / Hirotaka Onoe:1 
  • 1:理化学研究所 / Riken 2:京都大学 CiRA / Center for iPS Cell Research and Application (CiRA), Kyoto University 

Parkinson's disease is a progressive, degenerative neurological movement disorder that is resulted from the death of dopamine-generating cells in the substantia nigra. Stem cells, such as embryonic stem cell, induced pluripotent stem cell(iPSC), are expected for restoring lost function of dopaminergic neurons in Parkinson's disease.iPSC provide the potential for autologous transplantation using cells derived from a patient's own cells. To realize the transplantation therapy in human patients, it is quite important to know about the therapeutic effects of iPSC-derived dopaminergic neurons for Parkinson's disease in keeping with safety. We measured the function and growth of implanted iPSCs in the putamen of non-human primate using positron emission tomography(PET).PET scans with [11C]PK11195 and [11C](S)ketoprofen-methylester ([11C](S)KTP-Me) were performed with the use of an animal PET scanner to identify the TSPO and COX-1 of activation of microglia during the neuroinflammatory process, respectively. Immediately after the transplantation, increases in uptake of [11C]PK11195 and [11C](S)KTP-Me were observed in the sites of both allografts and autografts, while these were disappeared. However, increased uptake of both PET probes was reappeared at 3 months, especially in allografts at which [11C](S)KTP-Me showed more significant uptake than [11C]PK11195. These results indicate that PET imaging with [11C](S)KTP-Me might be useful for the precise early detection of immunogenicity in the clinical study with human patients.

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