• Top page
  • Timetable
  • Per session
  • Per presentation
  • How to
  • Meeting Planner



Stem Cells, Neuronal and Glial Production/Differentiation

開催日 2014/9/12
時間 14:00 - 15:00
会場 Poster / Exhibition(Event Hall B)

The sulfatide with short chain fatty acids is dominant in the oligodendrogenesis region of the embryonic spinal cord

  • P2-076
  • 和田(平原) 幸恵 / Yukie Wada-Hirahara:1 若林 毅俊 / Taketoshi Wakabayashi:1 森 徹自 / Tetuji Mori:1,3 小池 太郎 / Taro Koike:1 高森 康晴 / Yasuharu Takamori:1 矢尾 育子 / Ikuko Yao:2,4 後藤 仁志 / Hitoshi Gotoh:5 本家 孝一 / Koichi Honke:6 小野 勝彦 / Katsuhiko Ono:5 山田 久夫 / Hisao Yamada:1 
  • 1:関西医大・医・第一解剖 / Dept Anatomy and Cell Science, Kansai Medical University, Osaka, Japan 2:関西医大・医・医化学 / Dept Medical Chemistry, Kansai Medical University, Osaka, Japan 3:鳥取大・医 ・生体制御学 / Faculty of Medicine, Univ of Tottori, Tottori, Japan 4:浜松医大・医・基盤光医学 / Medical Photonics Research Center, Hamamatsu University School of Medicine, Shizuoka, Japan 5:京都府医大・生物学 / Dept Biology, Kyoto Prefectural University of Medicine, Kyoto, Japan  6:髙知大・医・生化学 / Dept Biochemistry, Kochi University Medical School, Kochi, Japan 

A pro-oligodendroblast-specific monoclonal antiboby O4 antigen named pro-oligodendroblast antigen (POA) has not been identified biochemically, whereas it has been widely used as a useful marker for this critical stage in the oligodendrocyte lineage. The O4 antibody reacts to sulfatide (HSO3-3-galactosylceramide) at the mature oligodendrocyte stage, but sulfatide synthesis at the pro-oligodendroblast stage is uncertain. In the present study we show by using imaging mass spectrometry that sulfatide exists at restricted regions of the ventricular zone of spinal cord in both of the stage 32 chick embryo and of the embryonic day 13 mouse fetus, where pro-oligodendroblasts first appear. There are various sulfatide isoforms with different fatty acid composition. We found that short-chain sulfatide with 18 and 20 carbon atoms (C18 and C20, respectively) are dominant during the initial appearance of pro-oligodendroblasts in the embryonic spinal cord, while long-chain (C24) sulfatide is dominant in adult spinal cord. However, in a later stage of oligodendrocyte maturation at mouse embryonic day 19, sulfatides with C22 or C24 become to be dominant. These results indicated that pro-oligodendroblasts have short-chain sulfatides and their fatty acid chain length changes with oligodendeocyte maturation stage and age. Moreover, O4-positive and O1 (anti-GalCer)-negative pro-oligodendroblasts were sorted using a flow cytometer and then their sulfatide forms were determined by LCMS-IT-TOF. The data show that the pro-oligodendroblasts have C18 sulfatide. Together, these results elucidate that POA at pro-oligodendroblast stage is the sulfatide with short chain fatty acids.

Copyright © Neuroscience2014. All Right Reserved.