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Super-resolution microscopy: nano-scale spotlight for discovery of new principle in brain operation

開催日 2014/9/13
時間 9:00 - 11:00
会場 Room C(502)
Chairperson(s) 林 康紀 / Yasunori Hayashi (独立行政法人理化学研究所 脳科学総合研究センター / RIKEN Brain Science Institute, Japan)
坂内 博子 / Hiroko Bannai (名古屋大学大学院理学研究科生命理学専攻 / Department of Biological Science, Graduate School of Science, Nagoya University, Japan)

Strategy for the self organization of plasma membrane revealed by quantum-dot single particle tracking

  • S3-C-1-2
  • 坂内 博子 / Hiroko Bannai:1,2 有薗 美沙 / Misa Arizono:2,4 丹羽 史尋 / Fumihiro Niwa:2 Triller Antoine / Antoine Triller:3 御子柴 克彦 / Katsuhiko Mikoshiba:2 
  • 1:名古屋大院理生命理学 / Dept. Biol. Sci., Nagoya Univ, Aichi, Japan 2:理研BSI発生神経生物 / Dev. Neurobiol., RIKEN BSI, Saitama, Japan 3:IBENS, INSERM U1024, Paris, France / IBENS, INSERM U1024, Paris, France 4:IINS, CNRS UMR 5297, Bordeaux, France / IINS, CNRS UMR 5297, Bordeaux, France 

According to the fluid mosaic model, plasma membrane molecules such as lipids and transmembrane proteins have the ability to undergo lateral diffusion freely throughout the cell. In some cell types, however, specific membrane molecules are concentrated in cellular microdomains, by overcoming the randomizing effects of free diffusion. This polarized distribution of membrane molecules is crucial for various cell types including neurons and astrocytes. Thus it is important to understand the mechanism through which the cell regulates the lateral diffusion of membrane molecules. Quantum-dot single particle tracking (QD-SPT), a super-resolution imaging technique using semiconductor nanocrystal quantum dots as a fluorescent probe, is a powerful tool to analyze the behavior of proteins and lipids on the plasma membrane. In this talk, we introduce the application QD-SPT to neuroscience. We report here that the lateral diffusion GABAA receptors, which plays important role in inhibitory synaptic transmission, is regulated by intracellular second messenger Ca2+. We also present a set of data indicating that the lateral diffusion barrier against metabotropic glutamate receptors (mGluRs) developed in astrocytes contributes to maintain compartmentalized distribution of mGluRs and the localized Ca2+ signal.

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