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Synaptic Plasticity

開催日 2014/9/11
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Phldb2 regulates the maturation of dendritic spines and synaptic plasticity

  • P1-057
  • 謝 敏かく / Min-Jue Xie:1,2,3 八木 秀司 / Hideshi Yagi:4 猪口 徳一 / Tokuichi Iguchi:5 岡 雄一郎 / Yuichiro Oka:5 黒田 一樹 / Kazuki Kuroda:1,3 柚崎 通介 / Michisuke Yuzaki:6 松田 信爾 / Shinji Matsuda:6 石川 保幸 / Yasuyuki Ishikawa:7 佐藤 真 / Makoto Sato:1,2,5,8 
  • 1:福井大・医・組織細胞形態学・神経科学領域 / Dept Morphol Physiol, Div Cell Biol Neurosci, Univ of Fukui, Fukui, Japan 2:福井大・子どものこころの発達研究センター / Res Center Child Mental Dev, Univ of Fukui, Japan 3:福井大・生命科学複合研究教育センター / Res Edu Program Life Sci, Univ of Fukui, Japan 4:兵庫医科大・医・解剖学・神経科学部門 / Dept Ana Neurosci, Hyogo College Med, Nishinomiya, Japan 5:大阪大学大学院・医・解剖学講座 / Dept Ana Neurosci, Osaka Univ Grad Sch Med, Osaka, Japan 6:慶應大・医・神経生理 / Dept Neurophysiol Sch Med, Univ of Keio, Tokyo, Japan  7:前橋工科大・システム生体工学 / Dept Sys Life Engineering, Maebashi Institu Tech, Maebashi, Japan 8:大阪大学大学院・連合小児発達学研究科・こころの発達神経科学講座 / United Grad Sch Child Dev, Osaka Univ, Japan 

Synapse function and plasticity depend on the morphology of dendritic spine. Dendritic filopodium is highly dynamic structure, known as a premature form of the dendritic spine. Here, we report that Phldb2 (pleckstrin homology-like domain, family B, member 2), one binding partner for a well-known actin-cross-linking protein Filamin A, works as a positive regulator of spine maturation. We generated Phldb2-knockout mice and found that a proportion of immature spines (filopodia and thin spines) increased in the hippocampus in vivo, which is consistent with our previous observations with Phldb2 knocked-down cultured hippocampal neurons. Next, we asked whether or not Phldb2 is involved in synaptic plasticity. To address this issue, we employed long-term potentiation (LTP) and Long-term depression (LTD) was induced in hippocampal slices by high frequency stimulation (HFS) and by low frequency stimulation (LFS), respectively. Recording field excitatory postsynaptic potentials (fEPSPs) were abolished in the Phldb2-knockout mice in both LTP and LTD. Therefore, Phldb2 mediates both LTP and LTD in hippocampus.

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