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Fear Memory

開催日 2014/9/13
時間 10:00 - 11:00
会場 Room J(313+314)
Chairperson(s) 森 憲作 / Kensaku Mori (東京大学大学院医学系研究科 細胞分子生理学分野 / Department of Physiology, Graduate School of Medicine, University of Tokyo, Japan)
三品 昌美 / Masayoshi Mishina (立命館大学 総合科学技術研究機構 脳科学 / Brain Science Laboratory, The Research Organization of Science and Technology, Ritsumeikan University, Japan)

Habenulo-raphe pathway underlies fear-motivated active avoidance behavior

  • O3-J-2-2
  • 天羽 龍之介 / Ryunosuke Amo:1,2 Fredes Felipe / Felipe Fredes:1 木下 雅恵 / Masae Kinoshita:1 青木 亮 / Ryo Aoki:1,3 相澤 秀紀 / Hidenori Aizawa:1 揚妻 正和 / Masakazu Agetsuma:1 青木 田鶴 / Tazu Aoki:1 白木 利幸 / Toshiyuki Shiraki:1 柿沼 久哉 / Hisaya Kakinuma:1 松田 勝 / Masaru Matsuda:4 山崎 昌子 / Masako Yamazaki:1 鷹架 美賀子 / Mikako Takahoko:1 東島 眞一 / Shin-ichi Higashijima:5 宮坂 信彦 / Nobuhiko Miyasaka:6 小出 哲也 / Tetsuya Koide:6 矢吹 陽一 / Yoichi Yabuki:6 吉原 良浩 / Yoshihir Yoshihara:6 岡本 仁 / Hitoshi Okamoto:1,2 
  • 1:理研BSI発生遺伝子制御 / Lab for Deveropmental Gene Regulation, RIKEN BSI, Saitama, Japan 2:早稲田大院先進理工生命医科 / Dept Life Sci and Medical Biosci, Waseda Univ, Tokyo, Japan 3:東京大院総合文化研 / Dept Life Sci, Graduate School of Arts and Sci, Univ of Tokyo, Tokyo, Japan 4:宇都宮大バイオサイエンス教育研究センター / Center for Bioscience Research and Education, Utsunomiya Univ, Tochigi, Japan 5:岡崎統合バイオサイエンスセ生理研 / NIPS, Okazaki Institute for Integrative Biosci, Aichi, Japan 6:理研BSIシナプス分子機構 / Lab for Neurobiology of Synapse, RIKEN BSI, Saitama, Japan 

Anticipation of danger at first elicits panic in animals but later it helps
them to avoid the real threat adaptively (e.g. active avoidance). The neural
circuit enabling such proactive use of danger expectation is unknown. A can
didate site responsible for active avoidance is the lateral habenula (LHb).
In mammals, LHb neurons are suggested to have a role in transmitting anti-re
ward and aversive information. The Hb is conserved in vertebrates, and the z
ebrafish homolog of the mammalian LHb, the ventral habenula (vHb) has a simp
le structure including an exclusive direct projection to the serotonergic me
dian raphe (MR). Using adult zebrafish as a model, we found that tetanus tox
in mediated genetic inhibition of synaptic transmission from the vHb to the
MR impaired active avoidance learning, while Pavlovian fear conditioning rem
ained intact. in vivo electrophysiolosy (loose-patch recording) from
genetically labeled vHb neurons during pavlovian fear conditioning showed th
at the vHb neuronal activity generated an expectation signal for a dangerous
context. Accordingly, artificially triggering an expectation signal by opto
genetic stimulation of vHb neurons in free-moving fish evoked place avoidanc
e. We also found that optogenetic stimulation of the vHb axons in acute slic
e activated genetically labeled serotonergic neurons in the raphe. Thus, the
vHb-MR circuit is essential for presenting danger expectation and programmi
ng active avoidance. These results reveal how perceived risk in the environm
ent is communicated to the serotonin system to avoid potential hazard.

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