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Stem Cells, Neuronal and Glial Production/Differentiation

開催日 2014/9/11
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Physiological meanings of the dynamics of progenitor cells for the maintenance of the ventricular zone

  • P1-071
  • 篠田 友靖 / Tomoyasu Shinoda:1 樋口 亮 / Ryo Higuchi:2 岡本 麻友美 / Mayumi Okamoto:1 長坂 新 / Arata Nagasaka:1 三浦 岳 / Takashi Miura:3 長山 雅晴 / Masaharu Nagayama:2 藤森 俊彦 / Toshihiko Fujimori:4 宮田 卓樹 / Takaki Miyata:1 
  • 1:名古屋大院医 / Dept Anatomy and Cell Biology, Nagoya Univ Graduate School of Medicine, Nagoya, Japan 2:北大・電子科学研究所 / RIES, Hokkaido Univ, Hokkaido, Japan 3:九州大院医 / Kyusyu Univ Graduate School of Medicine, Fukuoka, Japan 4:基生研 / Div Embryology, NIBB, Okazaki, Japan 

In the pseudostratified neuroepithelium (NE) or ventricular zone (VZ) of developing mammalian cerebral cortex, each neural progenitor cell shows nuclear movement along the apical-basal axis correlated with cell cycle called interkinetic nuclear migration (INM). While cell-autonomous molecular mechanisms were reported to be essential for INM, it was recently suggested that cell-to-cell physical interaction also play important role, especially for apical-to-basal INM of G1 cells. How INM of each progenitor cell is three-dimensionally coordinated to maintain the whole structure of the NE/VZ, however, still remains unclear. To answer the question, we performed: (1) Comprehensive monitoring of all NE/VZ cells by using H2B-mCherry or Lyn-Venus-transgenic mice. (2) 3D Mathematical simulation of INM as a group in NE/VZ. We found that: (1) the onset of basalward INM of the newborn G1 nuclei from the periventricular area was not always correlated with the presence of neighboring cellbodies, (2) the mode of the onset of basalward INM was completely different between newborn G1 cells pair. Our computational modeling also suggested that the active movement of newborn G1 nuclei in the periventricular area is indispensable for the continuous INM and the maintenance of the pseudostratified structure of NE/VZ. These observations suggest the existence of a novel mode of INM for newborn G1 cells, which may play critical roles for the maintenance of the characteristic structures of NE/VZ.

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