• Top page
  • Timetable
  • Per session
  • Per presentation
  • How to
  • Meeting Planner




開催日 2014/9/11
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Selective expression of glutamate receptor GluD1 at ascending pathway synapses in thalamic neurons of the ventral posteromedial nucleus

  • P1-039
  • 今野 幸太郎 / Kohtarou Konno:1 西川 晃司 / Koji Nishikawa:1 渡辺 雅彦 / Masahiko Watanabe:1 
  • 1:北海道大学 / Dept Anatomy & Embryology, Univ of Hokkaido, Sch Med, Sapporo, Japan 

Of the two members in the Δ family of ionotropic glutamate receptors, GluD1 is widely expressed in the adult brain, particularly higher brain regions. We have recently demonstrated that GluD1 works in concert with GluD2 for the construction of cerebellar synapse-connecting function (Konno et al., 2014). However, little is known to date regarding the expression and function of GluD1 outside the cerebellum. To address this issue, we examined the expression in the ventral posteromedial thalamic nucleus (VPM), a relay station in the trigeminal somatosensory pathway. Double-labeling fluorescence in situ hybridization addressed that GluD1 mRNA was expressed in glutamatergic thalamic neurons expressing vesicular glutamate transporter VGluT2 mRNA. VPM neurons are known to receive two types of glutamatergic inputs, one from VGluT2-positive input ascending from the trigeminal nuclei and another from VGluT1-positive input descending from the somatosensory cortex. By immunofluorescence, GluD1-positive clusters were closely apposed to VGluT2-positive terminals, but not VGluT1-positive terminals. Postembedding immunoelectron microscopy revealed that GluD1 was selectively localized on the postsynaptic membranes contacting VGluT2-positive terminals. Thus, GluD1 is selective to ascending pathway synapses in VPM neurons.

Copyright © Neuroscience2014. All Right Reserved.