• Top page
  • Timetable
  • Per session
  • Per presentation
  • How to
  • Meeting Planner




開催日 2014/9/11
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Maternal immune challenge in mice leads different gene expression and DNA methylation between male and female offspring

  • P1-363
  • 兪 志前 / Zhiqian Yu:1 舟山 亮 / Ryo Funayama:2 植野 和子 / Kazuko Ueno:3 成相 直樹 / Naoki Nariai:3 小島 要 / Kaname Kojima:3 小野 千晶 / Chiaki Ono:1 笠原 好之 / Yoshiyuki Kasahara:1 菊地 淑恵 / Yoshie Kikuchi:1 長崎 正朗 / Masao Nagasaki:3 中山 啓子 / Keiko Nakayama:2 富田 博秋 / Hiroaki Tomita:1,4 
  • 1:東北大学・災害研・災害精神医学分野 / Department of Biological Psychiatry, Tohoku University 2:東北大学・医・細胞増殖制御分野 / Cell Proliferation, Tohoku University 3:東北大学・メディカルメガバンク・バイオメディカル情報解析分野 / Biomedical Information Analysis, Tohoku University 4:東北大学・メディカルメガバンク / Tohoku Medical Megabank, Tohoku University 

Evidence suggests that both genetic and environmental factors are associated with schizophrenia. Maternal infection have been considered a risk factor for schizophrenia. Previous mouse studies showed that maternal immune challenge induced schizophrenia-related abnormal behavior in adults offspring. Postmortem studies have reported the alterations in gene expression and DNA methylation in schizophrenia. Taken together, maternal infection may influence genetic expression and DNA methylation during postnatal brain development, which involve in pathogeneses of schizophrenia. To investigate the transcriptional regulations affected by maternal immune challenge in fetal brain by microarray and ChIP-Seq, which remains throughout development and cause behavioral abnormality in adult. Poly I:C or PBS was administered into pregnant mice every 6 consecutive days from E14 to E19. The offspring were divided into 4 groups: Poly I:C-female, Poly I:C-male, PBS-female and PBS-male-mice. total RNA from prefrontal cortex of adults offspring was applied on Illumina Mouse WG-6 V2 Beadchips to analysis mRNA expression profiling. Methylated DNA was isolated from prefrontal cortex using MethylMiner Methylated DNA Enrichment Kit. Illumina Genome Analyzer IIx was used to analyze DNA methylation profiles. Sequenced reads were mapped to the mouse genome, and target genes-associated peak calls were determined with MACS and GREAT. Poly I:C-male-mice showed decreased %PPI only in 71 dB, whereas Poly I:C-female mice showed significantly decreased %PPI in all of 68, 71 and 77 dB. Specific gene expression profiles and methylation patterns were identified in both of Poly I:C-male and -female mice cam paring with its PBS-control, respectively. Poly I:C-female-mice showed more genes transcript alterations and DNA hypermethylation than male counterparts. Data suggest molecular mechanisms underlying the impact of maternal immune response to the fetal brain, which is preserved until adult and caused behavioral abnormality in gender-specific manner.

Copyright © Neuroscience2014. All Right Reserved.