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Reward and Decision Making

開催日 2014/9/11
時間 16:00 - 17:00
会場 Poster / Exhibition(Event Hall B)

Optogenetically evoked aversion via dopamine D2 receptors

  • P1-234
  • 壇上 輝子 / Teruko Danjo:1,2 吉見 建二 / Kenji Yoshimi:3 矢和多 智 / Satoshi Yawata:2 中西 重忠 / Shigetada Nakanishi:2 
  • 1:理化学研究所 / RIKEN Brain Science Institute 2:大阪バイオサイエンス研システムズ生物 / Department of Systems Biology, Osaka Bioscience Institute, Osaka, Japan  3:順天堂大学医学部 生理学第一講座 / Department of Neurophysiology, Juntendo University School of Medicine, Tokyo, Japan 

Dopamine (DA) transmission from the ventral tegmental area (VTA) is critical for controlling both rewarding and aversive behaviors. The transient silencing of DA neurons is one of the responses to aversive stimuli, but its consequences and neural mechanisms regarding aversive responses and learning have largely remained elusive. Here, we show that optogenetic inactivation of VTA DA neurons promptly down-regulated DA levels and induced up-regulation of the neural activity in the nucleus accumbens (NAc) as evaluated by Fos expression. This optogenetic suppression of DA neuron firing immediately evoked aversive responses to the previously preferred dark room and led to aversive learning toward the optogenetically conditioned place. The nucleus accumbens (NAc), the major output nuclei of VTA DA neurons, was considered to be responsible for this response, so we examined which of the fundamental pathways in the NAc was critical to this behavior by using knock-down of D1 or D2 receptor. This optogenetically evoked place aversion was abolished by knockdown of dopamine D2 receptors but not by that of D1 receptors in the NAc. Silencing of DA neurons in the VTA was thus indispensable for inducing aversive responses and learning through dopamine D2 receptors in the NAc.

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