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Learning and Long-term Memory

開催日 2014/9/12
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Imaging of the neural circuit activities during retrieval of behavioral program for active avoidance in zebrafish

  • P2-241
  • 柿沼 久哉 / Hisaya Kakinuma:1 青木 亮 / Ryo Aoki:1 青木 田鶴 / Tazu Aoki:1 山崎 昌子 / Masako Yamazaki:1 白木 利幸 / Toshiyuki Shiraki:1 鷹架 美賀子 / Mikako Takahoko:1 江泉 香里 / Kawori Eizumi:1 小出 哲也 / Tetsuya Koide:2 吉原 良浩 / Yoshihiro Yoshihara:2 中井 淳一 / Junichi Nakai:3 川上 浩一 / Koichi Kawakami:4 岡本 仁 / Hitoshi Okamoto:1 
  • 1:理研BSI発生遺伝子制御 / Lab. for Developmental Gene Regulation, RIKEN, BSI, Japan 2:理研BSIシナプス分子機構 / Lab. for Neurobiology of Synapse, RIKEN, BSI, Japan 3:埼玉大脳抹消科学研究センター脳機能解析部門 / Brain Science Institute, Saitama Univ., Japan 4:国立遺伝研個体遺伝初期発生 / Division of Molecular and Developmental Biology, NIG, Japan 

We have previously shown that the central part of the dorsal pallium (Dc) in zebrafish has an important role in recalling the long-term memories of behavioral programs for an active avoidance paradigm. This result suggests that in zebrafish, the pallio-basal ganglia circuit is required for reinforcement learning. Meanwhile it has been reported that the medial part of dorsal pallium (Dm), a presumptive homolog of mammalian amygdala, is involved in retention of the long-term memory of learned fear. Although both two areas, Dc and Dm, are required to recall the active avoidance, there have been little reports how these two areas are functionally or anatomically related.
In this study, using camk2a:GVP; UAS:G-CaMP7 line, we applied in vivo calcium imaging to the dorsal pallium to detect neural activities during retrieval of the behavioral program during the active avoidance. 24 hrs after last training, we measured florescence changes in response to LED stimulation as the CS presentation. In learner fish, neural activities in the Dm and the caudal Dc in addition to the Dc were observed upon the LED stimulation. The neural activities in the Dm preceded that in the Dc. In naive control fish, no signals were observed in these areas. These results suggest that neural activities in the Dc, the Dm and the caudal Dc are involved in a recall of the remote memory of behavioral paradigm.
Now we investigate a correlation of the neural activations among these three areas during a memory retrieval. We also try to measure and compare active neural areas related to different behavior paradigms such as the classical fear conditioning.

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