• Top page
  • Timetable
  • Per session
  • Per presentation
  • How to
  • Meeting Planner



Signal Transduction and Modulation

開催日 2014/9/12
時間 14:00 - 15:00
会場 Poster / Exhibition(Event Hall B)

Intracellular magnesium increase induced by neural activity in rat hippocampal neurons

  • P2-052
  • 山中 龍 / Ryu Yamanaka:1 新藤 豊 / Yutaka Shindo:1 苅部 尭応 / Takamasa Karube:1 棚元 亮 / Ryo Tanamoto:1 堀田 耕司 / Kohji Hotta:2 鈴木 孝治 / Kohji Suzuki:1 岡 浩太郎 / Kotaro Oka:1 
  • 1:慶應義塾大学 理工学研究科基礎理工学 / School of Fundamental, Sciecnce and Technology, Graduate School of Science and Technology, Keio University 2:慶應義塾大学 理工学研究科総合デザイン工学 / School of Integrated Design Engineering, Graduate School of Science and Technology, Keio University 

Magnesium ion (Mg2+) is involved in a wide variety of biochemical reactions, and a multitude of physiological functions are known to require Mg2+. Many previous works suggest that Mg2+ is involved in various neuronal diseases, such as migraine, Alzheimer's disease and Parkinson's disease. Therefore, Mg2+ dynamics in neurons may play key roles for maintaining normal neuronal functions. In facts, in neurons, main excitatory neurotransmitter glutamate induced intracellular Mg2+ concentration [Mg2+]i increase (Shindo et al. 2010). However, it has not been revealed whether neuronal activity under physiological condition modulate [Mg2+]i in neurons. This study aimed to explore the relationship between neural activity and [Mg2+]i dynamics.
To monitor [Mg2+]i dynamics during neural activation, we developed the combined system of neuronal stimulation and fluorescent imaging of cultured neurons. In concrete, we cultured rat hippocampal neurons on glasses coated with electrically conductive and optically transparent indium-tin oxide (ITO) films, and simultaneously visualized [Mg2+]i and [Ca2+]i dynamics by fluorescent dyes KMG-104-AM, a highly selective fluorescent Mg2+ probe, and fura red, a fluorescent Ca2+ probe, respectively, during neural stimulation through ITO electrodes.
We observed transient [Ca2+]i increase in response to stimulation, indicating our experimental system clearly stimulates enough to trigger neural activation, and we simultaneously detected the [Mg2+]i increase. Taken together, we concluded that neuronal activity accompanied the [Mg2+]i increase in rat hippocampal neurons.

Copyright © Neuroscience2014. All Right Reserved.