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開催日 2014/9/11
時間 16:00 - 17:00
会場 Poster / Exhibition(Event Hall B)

Inhibition of non-NMDA gulutamatergic transmission onto cholinergic neurons in the rat basal forebrain by serotonin

  • P1-046
  • 西條 琢真 / Takuma Nishijo:1 籾山 俊彦 / Toshihiko Momiyama:1 
  • 1:東京慈恵医大・薬理 / Dept Pharmacol, Jikei Univ Sch of Med, Tokyo, Japan 

The modulatory roles of serotonin in non-NMDA gulutamatergic transmission onto basal forebrain cholinergic neurons were investigated in P12-20 rat brain slices using whole-cell patch-clamp technique. Basal forebrain cholinergic neurons were identified with Cy3-192IgG injected into lateral ventricles 3-7 prior to the experiments. Pharmacologically isolated non-NMDA receptor-mediated excitatory postsynaptic currents were evoked by focal electrical stimulation. Bath application of serotonin at 10 or 30 μM inhibited the amplitude of the evoked excitatory postsynaptic currents by 28.1 ± 6.26% (n=6) and 41.0 ± 10.7% (n=6), respectively. A 5-HT1A receptor agonist, (R)-(+)-8-OH-DPAT (30 μM), and a 5-HT1B receptor agonist, CP93129 (30 μM), both also suppressed the excitatory postsynaptic currents. 5-HT-induced suppression of excitatory postsynaptic currents is significantly larger in low (1.0 mM) Ca2+-containing external solution, compared with in normal (2.4 mM Ca2+) Krebs solution. These results suggest that 5-HT inhibits non-NMDA glutamatergic transmission onto basal forebrain cholinergic neurons via 5-HT1A and 5-HT1B receptors, and that the inhibitory effect is saturated in the normal external solution.

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