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Cerebrovascular Disease and Ischemia

開催日 2014/9/13
時間 14:00 - 15:00
会場 Poster / Exhibition(Event Hall B)

Neuroprotective effects of lycopene against neuronal damage in the gerbil hippocampus induced by transient cerebral ischemia

  • P3-328
  • 藤田 公和 / Kimikazu Fujita:1 芳本 信子 / Nobuko Yoshimoto:2 今田 英己 / Hideki Imada:3 菅沼 大行 / Hiroyuki Suganuma:4 永田 豊 / Yutaka Nagata:1 宮地 栄一 / Eiichi Miyachi:1 
  • 1:藤田保健衛生大学 / Dept Physiol, Sch Med, Fujita Health Univ 2:名古屋文理大学短期大学部 食物栄養 / Dept. Nutrition and Food Sciences, Nagoya Bunri Univ. Col. Nagoya, Japan  3:東海学院大学 健康福祉学部 管理栄養 / Dept Registered Dietitians, Healtn and Welfar, Tokai Gakuin Univ. Kagamigahara, Japan 4:カゴメ株式会社総合研究所 / Research Institute KAGOME Co. Ltd. Tochigi, Japan 

Several days following transient ischemia/reperfusion treatment, apoptotic degenerative changes in hippocampal neurons in gerbil brain are observed. Cerebral ischemia increases the activity of astrocyte and microglia, and this cellular response may play a key role in brain injury development. In the present study, changes in astrocyte (GFAP) and microglia (Iba-1) in lycopene-induced neurodegenerative depression were investigated using Mongolian gerbil brains. In addition, we measured inflammatory cytokines (IL-1β and TNF-α) using ELISA. Animals were fed either a normal or a lycopene-containing diet (5 mg/100 g feed) freely. After attaining a body weight of 60-65 g, animals were subjected to transient cerebral ischemia for 10 min or a sham operation. Animals were decapitated under flothane anesthesia, and the brains were removed at intervals of 3h, 1, 3 and 7day post-treatment. GFAP and Iba-1 expression in the hippocampus were analyzed using Western blotting. Expression of GFAP and Iba-1 protein in the hippocampus gradually increased in control (ischemia and non-lycopene) group after ischemia/reperfusion. In contrast, GFAP and Iba-1 protein level was significantly lower in lycopene-treated ischemia group than the control group at 7d after ischemia. The results indicate that astrocyte and microglia activation decreased in the gerbil hippocampus due to lycopene and suggest that the oral administration of lycopene could protect neurons from ischemic damage.

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