• Top page
  • Timetable
  • Per session
  • Per presentation
  • How to
  • Meeting Planner



Olfaction, Taste, Chemical Senses

開催日 2014/9/13
時間 14:00 - 15:00
会場 Poster / Exhibition(Event Hall B)

Mice selectively bred for high and low glutamate consumption differ in vagus-mediated postingestive effects of glutamate

  • P3-140
  • 北村 明彦 / Akihiko Kitamura:1,2 井上 雅司 / Masashi Inoue:2,3 Nelson Theodore M / Theodore M Nelson:2 Theodorides Maria L / Maria L Theodorides:2 Bachmanov Alexander A / Alexander A Bachmanov:2 
  • 1:味の素株式会社 イノベーション研究所 / Inst for Innovation, Ajinomoto Co., Inc., Kanagawa, Japan 2:Monell Chemical Senses Center, Philadelphia, USA / Monell Chemical Senses Center, Philadelphia, USA 3:東京薬科大院生命科学脳神経機能 / Lab of cellular Neurobiol, Sch of Life Sci, Tokyo Univ of Pharm and Life Sci, Tokyo, Japan 

L-glutamate (Glu) plays multiple functions in the body. While Glu synthesized in the brain functions as a neurotransmitter, dietary free Glu stimulates taste receptors in the oral cavity to elicit umami taste sensation. After free or protein-bound Glu is ingested, it is metabolized and can evoke various physiological responses. Therefore, ingestive behavior towards Glu can be influenced by both its sensory and postingestive effects. In our previous study, we have found that inbred mouse strains differ in voluntary consumption of monosodium Glu (MSG). The goal of our subsequent studies has been to understand the genetic and physiological mechanisms of these differences in Glu appetite. To develop a better model for genetic and physiological studies of Glu appetite, we had intercrossed the C57BL/6ByJ and 129P3/J strains with large differences in voluntary MSG consumption, and then used selective breeding to produce mouse strains with high and low MSG intake (MSG-H and MSG-L, respectively). After 14 generations of selective breeding, MSG-H mice drank 6 times more 300 mM MSG than MSG-L mice. We used MSG-H and MSG-L mice to examine 1) behavioral and neural taste responses to amino acids and other tastants, 2) effect of MSG exposure on taste responses to MSG in brief-access tests , 3) flavor preferences conditioned by oral MSG, 4) blood glucose after MSG gavage, and 5) effect of vagotomy on MSG consumption in preference tests. We found that strain differences in MSG intake most likely depend on postingestive effects mediated by the vagus nerve. These data support the role of the vagus afferent nerve pathway in Glu appetite.

Copyright © Neuroscience2014. All Right Reserved.