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Neurodevelopmental Disorders

開催日 2014/9/13
時間 14:00 - 15:00
会場 Poster / Exhibition(Event Hall B)

Exposure of neonicotinoid pesticide disrupts gene expression profiles in cerebellar cultures from neonatal rats -possible causal factors for developmental disorders-

  • P3-314
  • 木村-黒田 純子 / Junko Kimura-Kuroda:1 西藤 泰昌 / Yasumasa Nishito:2 柳澤 比呂子 / Hiroko Yanagisawa:3 黒田 洋一郎 / Yoichiro Kuroda:4 小牟田 緑 / Yukari Komuta:5 川野 仁 / Hitoshi Kawano:6 林 雅晴 / Masaharu Hayashi:1 
  • 1:東京都医学研 こどもの脳プロジェクト / Mental Develop Project, Tokyo Metro Inst Med Sci, Tokyo, Japan 2:東京都医学研 基盤技術研究センター / Basic Tech Res Center, Tokyo Metro Inst Med Sci, Tokyo, Japan 3:東京都医学研 神経変性病理 / ALS/Neuropathy Project, Tokyo Metro Inst Med Sci, Tokyo, Japan 4:環境脳神経科学情報センター / Environ Neurosci Info Center, Tokyo, Japan 5:国立リハビリ研 視覚機能障害研究室 / Dept Rehab Sensory Function, NRCD, Saitama, Japan 6:帝京平成大 健康メディカル部 / Dept Health & Dietetics, Teikyo Heisei Univ, Tokyo, Japan 

Imidacloprid (IMI) and acetamiprid (ACE) belong to a new class of neonicotinoid pesticides, which are used widely in the world. They act as agonists on nicotinic acetylcholine receptors (nAChRs) and their high toxicities to insects are well studied, but their effects on mammalian nAChRs remain to be elucidated. Many types of nAChRs are important for not only neurotransmission, but also immune system and especially normal brain development. Previously we reported that ACE, IMI, and nicotine exert similar excitatory effects on rat cerebellar neurons (PLoS ONE 2012). In 2013, the European Food Safety Authority (EFSA) considered developmental neurotoxicity of these two neonicotinoids and recommended more stringent regulations. In this study, we examined gene expressions of three independent sets of rat cerebellar cells cultured for two weeks in the absence or presence of IMI, ACE, or NIC at concentrations of 1μM by DNA microarray (Agilent Whole Rat Genome Ver.3.0) and analytical software (Gene Spring GX, Agilent). From a total of 30,367 DNA probes (26,930 gene RNAs) in the DNA array, about 25,000 mRNA transcripts were detected. Between IMI, ACE, or nicotine-treated cultures versus control (C), 247 transcripts (IMI vs. C), 175 transcripts (ACE vs. C), or 100 transcripts (NIC vs. C) showed statistically significant differences (moderate T test, P<0.05) at least 1.5 fold, which include important genes for brain development. Microarray data for selected genes were confirmed by quantitative real-time PCR. The results suggest that neonicotinoids disrupt gene expressions in the developing brain, and are possible causal factors for developmental disorders.

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