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Cell Migration and Layer/Nuclear Formation

開催日 2014/9/12
時間 16:00 - 17:00
会場 Room J(313+314)
Chairperson(s) 見学 美根子 / Mineko Kengaku (京都大学 物質-細胞統合システム拠点 / Instituete for Integrated Cell-Material Sciences(iCeMS), Kyoto University, Japan)
前田 信明 / Nobuaki Maeda (公益財団法人東京都医学総合研究所神経回路形成プロジェクト / Neural Network Project, Tokyo Metropolitan Institute of Medical Science, Japan)

The unique migratory properties of glial progenitors derived from the cortical ventricular zone

  • O2-J-4-1
  • 田畑 秀典 / Hidenori Tabata:1,2 佐々木 恵 / Megumi Sasaki:2 竹林 浩秀 / Hirohide Takebayashi:3 依馬 正次 / Masatsugu Ema:4 池中 一裕 / Kazuhiro Ikenaka:5 永田 浩一 / Koh-ichi Nagata:1 仲嶋 一範 / Kazunori Nakajima:2 
  • 1:愛知県心身障害者コロニー発達障害研 / Dept of Mol Neurobiol, Inst for Dev Res, Aichi Human Service Cent, Aichi, Japan 2:慶應大・医・解剖 / Dept Anat, Sch of Med, Keio Univ, Tokyo, Japan 3:新潟大院・医歯学総合・神経生物 / Div of Neurobiol & Anat, Grad Sch of Med & Den Sci, Niigata Univ, Niigata, Japan  4:滋賀医大・動物生命科学研究セ / Res Cent for Animal Life Sci, Shiga Univ of Med Sch, Shiga, Japan 5:生理研・分子神経 / Div of Neurobiol & Bioinformatics, NIPS, Aichi, Japan 

Although the migration behaviors of neurons have been extensively observed and described, the development of glial cells has not been fully understood. We have established in utero electroporation system, and by using this system we visualized the migrating cells derived from the cortical ventricular zone (VZ), and observed the migratory behaviors of them in detail. During these observations, we have noticed a novel population that moves in an unreported irregular manner. These cells frequently change its direction and rate of migration, and they were observed to divide in the IZ. We named this migration mode "erratic migration". To identify the fate of erratic migrating cells, we performed dissociation culture after specific labeling of them by using photoconversion of kikGR during time-lapse observations. We found that the cells that had assumed erratic migration in the slice culture mostly formed colonies of GFAP-positive astrocytes (88%). Moreover, the immunohistochemistry on the slices after time-lapse observations revealed that about 80% of the erratic migrating cells were positive for Olig2, a marker for glial progenitors. These observations indicated that the erratic migrating cells were glia progenitors that mainly produced astrocytes. The fate analysis using Olig2CreER mice revealed that the cortical VZ derived Olig2 expressing cells, which assumed erratic migration in slice culture, mainly differentiated into astrocytes distributed widely in the cortical gray matter. On the other hand, the VZ cells labeled by electroporation after birth (on the day of birth or postnatal day 1) produced few or no erratic migrating cells, and mainly differentiated into astrocytes in the white matter or deep pert of the gray matter.

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