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Mood Disorders

開催日 2014/9/12
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Analysis of FosbF/F mice expressing only FOSB and vFOSB but not ΔFOSB and Δ2ΔFOSB

  • P2-337
  • 加藤木 敦央 / Atsuhisa Katogi:1 能丸 寛子 / Hiroko Nomaru:1 大西 克典 / Yoshinori N. Ohnishi:1 作見 邦彦 / Kunihiko Sakumi:1,2 中別府 雄作 / Yusaku Nakabeppu:1,2 
  • 1:九大 生医研 脳機能制御学 / Div. Neurofunc. Genomics, MIB., Kyushu Univ., Fukuoka, Japan 2:九大 ヌクレオチドプール研究センター / Res. Ctr. Nucleotide pool, Kyushu Univ., Fukuoka, Japan 

Activator protein-1 (AP-1) transcription factor is composed of JUN family proteins and FOS family proteins. Among Fos family genes, only Fosb gene produces two forms of mature transcripts, Fosb and ΔFosb mRNAs by alternative splicing. By alternative translation initiation, the former encodes full-length FOSB and vFOSB, while the latter encodes ΔFOSB and Δ2ΔFOSB both of which lack the C-terminal 101 amino acids of FOSB. We previously reported that Fosb-null mice show anxiety-like behavior, depressive-like behavior and adult-onset epilepsy, while Fosbd/d mutant mice expressing only ΔFOSB and Δ2ΔFOSB do not show such abnormal behaviors. However, it is still unclear whether FOSB and/or vFOSB have some roles in these abnormal behaviors. To further understand how FOSB and/or ΔFOSB regulate such behaviors, we established FosbF/F mice expressing only full-length Fosb mRNA. By western blotting, we confirmed that only FOSB and vFOSB proteins, which are not detected in Fosbd/d and Fosb-null mice, are detected in whole cell extracts prepared from FosbF/F mouse brains. We next performed a series of behavioral tests including open-field test, elevated plus maze test, repeated forced swim test and home-cage activity measurement. In elevated plus maze test, FosbF/F mice exhibited significantly decreased time spent on an open arm compared with wild-type littermates, as observed in Fosb-null mice. However, the immobility time during forced swim test and spontaneous locomotor activity were not changed compared with wild-type littermates. Moreover, FosbF/F mice did not exhibit adult-onset epilepsy. In summary, FosbF/F mice exhibited anxiety-like behavior, as seen in Fosb-null mice, but not depressive-like behavior nor adult-onset epilepsy. We thus concluded that FOSB and/or vFOSB are enough to suppress depressive-like behavior and adult-onset epilepsy, but not enough to suppress anxiety-like behavior.

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