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Synaptic Plasticity

開催日 2014/9/12
時間 14:00 - 15:00
会場 Poster / Exhibition(Event Hall B)

Effect of chronic fluoxetine on the morphology of perforant path synapse in the dentate gyrus : 3D ultrastructural analyses using FIB/SEM

  • P2-048
  • 北原 陽介 / Yosuke Kitahara:1 太田 啓介 / Keisuke Ohta:2 首藤 隆秀 / Takahide Shuto:1 黒岩 真帆美 / Mahomi Kuroiwa:1 外角 直樹 / Naoki Sotogaku:1 蓮尾 博 / Hiroshi Hasuo:3 都合 亜記暢 / Akinobu Togo:4 中村 桂一郎 / Kei-ichiro Nakamura:2 西 昭徳 / Akinori Nishi:1 
  • 1:久留米大・医・薬理 / Dept. of Pharmacol., Kurume Univ. Sch. of Med, Kurume, Fukuoka , Japan 2:久留米・医・第二解剖 / Dept. of Anat., Kurume Univ. Sch. of Med, Fukuoka, Japan 3:久留米大・医・生理・統合自律機能 / Dept. of Physiol., Kurume Univ. Sch. of Med, Fukuoka , Japan 4:久留米大・医・電子顕微鏡室 / Center Unit of Electron Microscopy, Kurume Univ. Sch. of Med, Kurume, Japan 

Chronic fluoxetine treatment increases the excitability of mature granule cells (GCs) in the dentate gyrus (DG), in addition to the stimulation of neurogenesis. Perforant path axons (boutons) from the entorhinal cortex to DG form synapses on dendritic spines of GCs, known as perforant path synapse. Electrophysiological analysis using voltage sensitive dye detected the enhanced excitatory neurotransmission of perforant path synapse in the middle molecular layer (MML) of DG after chronic fluoxetine treatment. In this study, we investigated the effect of chronic fluoxetine (15 mg/kg/day, 14 days) on the morphology of perforant path synapse in the MML by focused ion beam (FIB)/scanning electron microscopy (SEM). 3D ultrastructural analyses of dendritic spine revealed the appearance of the extremely large-sized spine after chronic fluoxetine, which was confirmed with quantification of spine volume. However, chronic fluoxetine did not affect volume distribution of the regular-sized spine or spine density. Sizes of postsynaptic density at the large-sized spine and of presynaptic bouton connected to the large-sized spine were large in volume. These results demonstrate that chronic fluoxetine treatment induces the large-sized perforant path synapse, which may be involved in the increased glutamatergic neurotransmission from perforant path to GCs.

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