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脳血管障害と虚血 1
Cerebrovascular Disease and Ischemia 1

開催日 2014/9/13
時間 17:10 - 18:10
会場 Room I(311+312)
Chairperson(s) 八木田 佳樹 / Yoshiki Yagita (川崎医科大学脳卒中医学教室 / Department of Stroke Medicine, Kawasaki Medical School, Japan)
山内 浩 / Hiroshi Yamauchi (滋賀県立成人病センター研究所 / Shiga Medical Center Research Institute, Japan)

Development of less invasive transient middle cerebral artery occlusion model on Common Marmoset and transplantation of NS/PCs

  • O3-I-5-1
  • 井上 賢 / Satoshi Inoue:1,2,3 原 晃一 / Kohichi Hara:4 岩井 宏樹 / Hiroki Iwai:5 小牧 裕司 / Yuji Komaki:3,6 岩田 祐士 / Hiroshi Iwata:7 塚田 秀夫 / Hideo Tsukada:8 中村 雅也 / Masaya Nakamura:5 岡野 ジェイムス洋尚 / Hirotaka James Okano:9 吉田 一成 / Kazunari Yoshida:2 岡野 栄之 / Hideyuki Okano:3 
  • 1:東京歯科大学市川総合病院 / Neurosugery, Tokyo Dental College Ichikawa General Hospital, Japan 2:慶應義塾大学医学部 脳神経外科 / Dept Neurosurgery, Univ of Keio, Tokyo, Japan 3:慶應義塾大学 生理学教室 / Dept Physiol, Univ of Keio, Tokyo, Japan 4:日野市立病院 脳神経外科 / Hino Municipal Hospital, Tokyo, Japan 5:慶應義塾大学医学部整形外科 / Dept Orthopaedic surgery, Univ of Keio, Tokyo, Japan 6:実験動物中央研究所 / Central Institute for Experimental Animals, Kawasaki, Japan 7:島津製作所 / Shimadzu Corporation, Kyoto, Japan 8:浜松ホトニクス 中央研究所 PETセンター / Hamamatsu Photonics K.K. PET Center, Hamamatsu, Japan 9:東京慈恵会医科大学 再生医学研究 / Regenerative Medicine ,Univ. of Jikei, Tokyo, Japan 

[Objective] We have developed a less invasive transient middle cerebral artery occlusion (MCAO) model on primates, common marmosets, without invasive procedure including craniotomy. Behavioral assessments (motor paresis, sensory disturbance and visual field defect) , MRI and positron emission tomography (PET) were performed with time postoperatively. And then, we checked whether our model would be useful for cell therapy. [Method] Female laboratory-bred common marmosets were used. Under anesthesia, wire thread was inserted into right MCA via carotid artery. We used 3 hours occlusion. Behavioral assessments, MRI and PET were performed postoperatively. In PET study, we used BCPP-EF, which is ligand to the complex1 of mitochondria. Transplanted cells were neural stem cells or precursor cells of a marmoset embryo brain. [Result] At postoperative day 7, MRI T2 weighted image showed brain infarction on right hemisphere including basal ganglia as high intensity lesion. However, after 28 days, the high intensity area was reduced dramatically. In PET, BCPP-EF showed the comparable result. Behavioral tests exhibited neurological deficits with left hemiparesis deteriorating for 4 days after surgery. After that, neurological function improved gradually. Six weeks after surgery, the behavior did not recover completely. Remained motor paresis such as ham hand could be observed by food retrieval test. The immunohistochemical study also showed defect of neural cells on the lesion. There were no complications by transplant. Transplanted cells could be checked by PET. [Conclusion] We have developed less invasive marmoset MCAO model. MRI, PET and behavioral tests showed reasonable brain infarction as well as human. The model is considered to be useful to evaluate the effect of the cell therapy of the cerebral infarction of the primates.

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